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基于HTP标记对玉米DH、RIL和BC群体遗传规律的比较分析
Comparative Analysis of Genetic Laws of Maize DH, RIL and BC Populations Based on HTP Markers
【摘要】 以先玉335双单倍体(Doubled Haploids,DH)群体、掖107×昌7-2重组自交系(Recombined inbred lines,RIL)群体和京724×京92回交1代(Backcross,BC)群体为试材,采用全基因组覆盖的新型区块标记——单倍型标签多态性(haplotype-tag polymorphisms,HTP)标记,从基因型分离比、亲本遗传片段和重组交换3个角度解析DH、RIL、BC群体的遗传规律。结果表明,DH群体虽然偏分离高于BC群体,平均交换频率低于RIL群体,但该群体样本中不存在杂合和外源片段,均为纯合片段;平均亲本遗传片段长度为384个HTP,高于RIL群体;单代上拥有最高的重组交换次数,相较于其他群体,在群体特点和创制成本等方面具有较多优势。
【Abstract】 In this study, HTP markers were used to analysis the genetic laws of Xianyu335 DH population,Ye107×Chang7-2 RIL population and Jing724×Jing 92 population, and Jing724×Jing 92 BC1 population from three perspectives: genotype segregation ratio, parental genetic fragmentation, and recombination exchange. The reesults showed that the average exchange frequency of DH population is lower than that of the RIL population, and no heterozygous and exogenous fragments. The average parental genetic fragment length is 384 HTP higher than RIL population, and it has the highest number of recombination exchanges in a single generation. Compared to BC1 and RILpopulation the DH has more advantages in terms of population characteristics and creation costs.
- 【文献出处】 玉米科学 ,Journal of Maize Sciences , 编辑部邮箱 ,2024年02期
- 【分类号】S513
- 【下载频次】21