【摘要】 旨在明确甘肃省草地贪夜蛾的入侵来源，并制定科学有效的防控对策。基于mtCOI基因分子标记分析中国草地贪夜蛾不同生态区8个省12个地理种群276个样品的遗传多样性指数、遗传分化系数及基因流等。结果表明，甘肃省草地贪夜蛾种群的单倍型多样性指数和平均核苷酸差异数分别为0.133～0.157与0.133～0.317,均低于中国周年繁殖区广东、广西、云南种群的0.157～0.819与1.033～7.705;所有种群的Tajima’s D中性检验和Fu’s F检验结果均为负值，表明草地贪夜蛾入侵中国后经历了明显的种群扩张事件。四川种群与其他种群遗传分化显著，62个种群间存在中等程度以上的基因交流。陕西略阳、陕西宁强、甘肃徽县、甘肃成县种群的有效迁入个体数和有效迁出个体数之和分别为11 860.66、11 708.65、10 878.66和10 379.32,在中国草地贪夜蛾的基因交流过程中具有中继站的作用，表明陕南汉水谷地为中国草地贪夜蛾西线北迁入侵西北的主要通道。更多还原

【Abstract】 This study aims to elucidate the invasion source and develop scientifically sound and effective prevention and control measures for Spodoptera frugiperda in Gansu province.Using molecular markers based on mtCOI gene, we analyzed the genetic diversity index, genetic differentiation coefficient and gene flow of S.frugiperda across 12 geographical populations in 8 provinces of China.The results showed that the haplotype diversity and average nucleotide difference in Gansu populations ranged from 0.133 to 0.157 and 0.133 to 0.317,respectively.These values were lower than thoose observed in Guangdong, Guangxi, and Yunnan populations within the annual breeding areas of China, which ranged from 0.157 to 0.819 and 1.033 to 7.705 respectively.Both Tajima’s D neutral test and Fu’s F test yielded negative values across all populations, suggesting a significant population expansion event for S.frugiperda following its invasion of China.There was genetic differentiation between Sichuan population and others, while gene flow among 62 populations were found to be at an above-medium level.The cumulative effective emigration and immigration values for the Lueyang, Ningqiang, Huixian county and Chengxian county populations were 11 860.66,11 708.65,10 878.66 and 10 379.32,respectively.These play the role as relaying station in the process of gene exchange of S.frugiperda in China.The Hanshui Valley is an important corridor for the westward migration of S.frugiperda into the northwest in China.更多还原