【摘要】 [目的]水稻纹枯病是水稻主要病害之一，严重影响水稻的产量。宁南霉素（ningnanmycin）可有效防治多种农作物病害。[方法]测定了在感染纹枯病的水稻上喷施宁南霉素的生理生化指标；通过qRT-PCR分析了病程相关蛋白相关基因PR1-α、PR-3和PR-5的表达。[结果]与Rhizoctonia solani组相比，ningnanmycin+Rhizoctonia solani组水稻叶片的叶绿素a、叶绿素b、类胡萝卜素含量和β-1,3-葡聚糖酶、内切几丁质酶、PPO的活性在4～7 d显著增加；MDA、O₂^-和H₂O₂的含量在3～7 d显著降低；PAL、SOD、POD和CAT的活性在3～7 d显著增加；ningnanmycin+Rhizoctonia solani组水稻叶片的OsPR1-α、OsPR-3和OsPR-5基因的相对表达量在3～7 d显著高于Rhizoctonia solani组。[结论]宁南霉素通过诱导产生病程相关蛋白和保护酶，增强植株寄主抗性，可有效控制立枯丝核菌引起的水稻纹枯病。更多还原

【Abstract】 [Aims] Rice（Oryza sativa L.） sheath blight is one of the major diseases of rice, seriously harming rice yield. Ningnanmycin can effectively prevent and control a variety of crop diseases. [Methods] This study measured the physiological and biochemical indicators on rice infected with Rhizoctonia solani after spraying ningnanmycin; the expression of disease course-related protein-related genes PR1-α, PR-3 and PR-5 was analyzed by qRT-PCR.[Results] Compared with the Rhizoctonia solani group, the contents of chlorophyll a, chlorophyll b, total carotenoid and the activities of β-1,3-glucanase, endochitinase and PPO in rice leaves of the ningnanmycin+R. solani group were significantly higher on day 4 to 7; the content of MDA, O₂^-, and H₂O₂ significantly decreased from day 3 to day 7; the activities of PAL, SOD, POD, and CAT significantly increased from day 3 to day 7; the relative expression of OsPR1-α,OsPR-3 and OsPR-5 in rice leaves of the ningnanmycin+R. solani group were significantly higher than those of the R. solani group from day 3 to day 7. [Conclusions] Ningnanmycin can effectively control rice sheath blight caused by R. solani by inducing the production of disease-related proteins and protective enzymes to enhance plant host resistance.更多还原