【摘要】 目的 探讨核受体NURR1过表达对前列腺癌(PCa)细胞中环状RNA(circRNA)表达谱的影响，为揭示NURR1相关circRNA分子在PCa进展过程中的作用提供参考。方法 通过UALCAN和TNMplot分析NURR1在PCa中的表达；通过高通量测序分析筛选NURR1过表达的PCa细胞中特征性circRNA分子表达谱；通过GO和KEGG分析差异表达的circRNA分子的功能和参与的信号通路。结果 circ＿0000915在DU145、LNCaP以及PC3细胞中均发生显著的下调表达。在核受体NURR1上调表达的DU145细胞中，1个circRNA上调表达(circ＿0005991),2个circRNA下调表达(circ＿0001460、circ＿0001315);在核受体NURR1上调表达的LNCaP细胞中有2个circRNA显著上调表达(circ＿0040729、circ＿0000722);在NURR1上调表达的PC3细胞中有3个circRNA上调表达(circ＿0001577、circ＿0000854、circ＿0018168),4个circRNA下调表达(circ＿013035、circ＿0003028、circ＿0082096、circ＿0005320)。KEGG分析发现差异表达的circRNA分子与背侧/腹侧神经管模式、蛋白质折叠伴侣、无序结构域特异性结合、BMP信号通路的正调控以及神经管模式化功能显著相关。结论 circRNA在NURR1介导的PCa进展过程中发挥着重要作用，但是在不同的PCa细胞类型中存在着一定的差异。核受体NURR1与circ＿0000915分子在PCa进展中的分子机制有待进一步研究。更多还原

【Abstract】 Objective To investigate the expression profile of circRNA in nuclear receptor NURR1 overexpressed prostate cancer(PCa) cells, so as to provide reference for revealing the mechanism of PCa progression.Methods The expression of NURR1 in PCa was analyzed with UALCAN and TNMplot. The distinct circRNAs in NURR1 overexpressed PCa cells were screened with RNA-sequencing. The functions and signal pathways of differentially expressed circRNA molecules were analyzed with GO and KEGG. Results The circ＿0000915 was significantly downregulated in DU145,LNCaP and PC3 cells. In NURR1 overexpressed DU145 cells, circ＿0005991 was up-regulated, while circ＿0001460 and circ＿0001315 were down-regulated. In NURR1 overexpressed LNCaP cells, circ＿0040729 and circ＿0000722 were significantly up-regulated. In NURR1 overexpressed PC3 cells, circ＿0001577,circ＿0000854 and circ＿0018168 were up-regulated, while circ＿013035,circ＿0003028,circ＿0082096 and circ＿0005320 were down-regulated. KEGG analysis revealed that the differentially expressed circRNAs were significantly associated with dorsal/ventral neural tube patterns, protein folding chaperones, disordered domain specific binding, positive regulation of BMP signaling pathways, and neural tube patterning functions. Conclusion CircRNAs play an important role in NURR1 mediated PCa progression, but there are certain differences among different prostate cancer cell types. The regulatory mechanism between NURR1 and circ＿0000915 in the progression of PCa needs further investigation.更多还原