【摘要】 带3蛋白是红细胞膜上一种重要的通道蛋白，介导细胞膜内外的阴离子转运过程，同时有助于红细胞形态的维持，具有重要的生理功能。然而，带3蛋白在原生细胞膜上的分布状态目前尚不清楚。冷冻扫描电子显微镜可在近生理状态下对生物样品的表面形貌进行成像。为了探究生理状态下带3蛋白在红细胞膜上的分布情况，本研究利用带3蛋白抗体修饰5 nm的金纳米颗粒，与人血红细胞膜上的带3蛋白特异性结合，并通过冷冻扫描电子显微镜进行成像，得到近生理条件下带3蛋白在人血红细胞膜上的分布图谱。结果表明，红细胞膜上的膜蛋白倾向于成簇分布，形成“蛋白岛”，而带3蛋白主要分布在这些蛋白岛上，彼此之间紧密连接，形成若干个功能微区发挥作用。更多还原

【Abstract】 Band 3 protein is an important channel protein in the erythrocyte membrane which mediates the anion transport process inside and outside the cell membrane,as well as contributes to the maintenance of erythrocyte morphology,and has important physiological functions.However,the distribution state of this protein in the primary cell membrane is not known.Cryo-scanning electron microscopy enables imaging of the surface morphology of biological samples in a near-physiological state.In order to investigate the distribution of band 3 protein on erythrocyte membranes under physiological conditions,the present study utilized 5-nm gold nanoparticles modified with the antibodies to specifically bind to the band 3 protein on human blood erythrocyte membranes and imaged them by cryo-scanning electron microscopy,to obtain distribution of band 3 protein on human blood erythrocyte membranes.The results showed that the membrane proteins on the erythrocyte membranes tended to be clustered and distributed to form″protein islands″,and band 3 proteins were mainly distributed in these protein islands,which were tightly connected with each other to form several functional microregions to play their respective roles.更多还原