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盐度胁迫对褐菖鲉幼鱼消化酶和非特异性免疫酶的影响

Effects of salinity on digestive enzyme and non-specific immune enzyme of juvenile Sebastiscus marmoratus

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【作者】 蒋飞徐嘉波林琪施永海贾垂攀严银龙邓平平于爱清张之文袁新程

【Author】 JIANG Fei;XU Jiabo;LIN Qi;SHI Yonghai;JIA Chuipan;YAN Yinlong;DENG Pingping;YU Aiqing;ZHANG Zhiwen;YUAN Xincheng;Shanghai Fisheries Research Institute, Shanghai Fisheries Technical Extension Station;Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province;Fisheries Research Institute of Fujian;

【通讯作者】 施永海;

【机构】 上海市水产研究所,上海市水产技术推广站福建省海洋生物增养殖与高值化利用重点实验室福建省水产研究所

【摘要】 为探讨褐菖鲉(Sebastiscus marmoratus)幼鱼适应盐度骤变的能力,本文以肠胃消化酶和肝脏非特异性免疫酶活性为指标,研究其在盐度6、8、10和20[分别记为S6、S8、S10和S20(对照)]胁迫下的变化规律。结果显示,当盐度胁迫96 h时,S10和S20组的胰蛋白酶、胃蛋白酶、淀粉酶和脂肪酶活性均显著高于S6和S8组(P<0.05);S6和S8组谷丙转氨酶和谷草转氨酶活性均显著高于S10和S20组(P<0.05);S6和S8组酸性磷酸酶和碱性磷酸酶活性均无显著性差异(P>0.05);S10和S20组酸性磷酸酶和碱性磷酸酶活性均呈先上升后下降趋势,均在12 h时达到峰值,且显著高于其他组。研究结果表明,褐菖鲉幼鱼适宜的养殖盐度为10~20。

【Abstract】 To investigate the changes in digestive enzyme activities in the intestine and stomach, as well as the activities of non-specific immunoenzymes in the liver of juvenile Sebastiscus marmoratus under salinity stress, and to assess the ability of the juveniles to adapt to sudden changes in salinity, a salinity experiment was conducted. The experiment comprised four groups, representing different salinity gradient levels: 6, 8, 10, and 20(control). These groups were referred to as S6, S8, S10, and S20, respectively. Results indicated that at the acute stress period of 96 hours, the activities of trypsin, pepsin, amylase, and lipase in S10 and S20 were significantly higher compared to those in S6 and S8(P<0.05). Conversely, the activities of glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase were significantly higher in S6 and S8 compared to S10 and S20(P<0.05). There was no significant difference in the activities of ACP and AKP between S6 and S8(P>0.05). Additionally, the activities of ACP and AKP increased initially and then decreased over time in S10 and S20. These activities in S10 and S20 peaked at 12 hours, signifying a significant increase compared to other salinity levels. These findings suggest that the optimal salinity for breeding juvenile S.marmoratus ranges from 10 to 20.

【基金】 福建省海洋生物增养殖与高值化利用重点实验室开放课题项目(2021fjscq02);上海领军人才项目(沪人社专2021-67号);上海长江口主要经济水生动物人工繁育工程技术研究中心(13DZ2251800)
  • 【文献出处】 渔业研究 ,Journal of Fisheries Research , 编辑部邮箱 ,2024年02期
  • 【分类号】S965.3
  • 【下载频次】92
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