【摘要】 为探究低聚果糖(fructo-oligosaccharides, FOS)、低聚半乳糖(galacto-oligosaccharide, GOS)以及FOS与GOS组合物(FOS+GOS)对小鼠脾淋巴细胞增殖活性、辅助型T细胞(helper T cell, Th细胞)分化的影响，以及对Th1/Th2平衡的调节作用，采用FOS、GOS和FOS+GOS分别孵育细胞，通过MTT比色法检测小鼠脾淋巴细胞增殖水平，通过酶联免疫吸附和PCR技术测定Th1、Th2型细胞因子及转录因子T-bet、GATA-3的表达情况，明确Th细胞分化方向。FOS、GOS和FOS+GOS对小鼠脾淋巴细胞的增殖活性的影响与其添加浓度相关，125μg/mL质量浓度的FOS、GOS和FOS+GOS均能提高脾淋巴细胞增殖活性，其中FOS+GOS对脾淋巴细胞增殖的促进作用最强。在无刀豆蛋白A(concanavalin A, ConA)刺激时，FOS+GOS对脾淋巴细胞增殖活性的促进作用最强；在有ConA刺激下，几种处理对脾淋巴细胞增殖活性的作用差异不显著。GOS和FOS+GOS处理均能够提高Th1型细胞因子IFN-γ的分泌量以及IFN-γ和IL-2 mRNA的表达水平，并能抑制Th2型细胞因子IL-4的分泌量以及IL-5 mRNA的表达水平，增加T-bet/GATA-3的比率。FOS+GOS与GOS单独处理均具有免疫调节活性，并能调节Th1/Th2平衡，具有潜在的抗过敏功效。更多还原

【Abstract】 The aim of the present study was to investigate the effects of fructo-oligosaccharides(FOS), galacto-oligosaccharide(GOS), and the mixture of FOS and GOS(FOS+GOS) on the proliferation of splenic lymphocytes, as well as the regulation of Th cell differentiation and Th1/Th2 balance. Spleen lymphocytes of mice were incubated with FOS. GOS and FOS+GOS mixture, respectively, with or without Concanavalin A(ConA) stimulation. Spleen lymphocyte proliferation level wasdetected by MTT colorimetric assay. To explore the differentiation direction of Th cell, the expression ofThl and Th2 cytokines and the transcription factors T-bet and GATA-3 were determined by enzyme linked immunosorbent assay(ELISA) and PCR, respectively. The results showed that the proliferative activities of FOS, GOS and FOS+GOS mixture on mouse splenic lymphocytes were concentration-dependent, and all of them exhibited promoting splenic cell proliferation activity at the concentration of 125 μg/mL, with FOS+GOS mixture having the strongest promotion effect on splenic cell proliferation. There was no significant difference among them on the splenic cell proliferation activity under ConA stimulation. FOS+GOS mixture had the strongest effect on the splenic cell proliferation activity without ConA stimulation. The supplementation of GOS and FOS+GOS mixture increased the secretion of Th1-type cytokine IFN-γ and the mRNA expression levels of IFN-γ and IL-2, and inhibited the expression and secretion levels of Th2-type cytokine-related mRNA including IL-4 and IL-5. In conclusion, both FOS+GOS mixture and GOS possess immunoregulatory activity and regulate Th1/Th2 balance, which deserve them as potential anti-allergic ingredients.更多还原