【摘要】 [目的]筛选出中国虎纹蛙高质量多态性的微卫星标记，用于该物种的鉴定以及遗传多样性、种群结构、遗传连锁图谱分析。[方法]利用新一代测序技术，从中国虎纹蛙部分基因组中筛选出33个三核苷酸重复、42个四核苷酸重复和1个五核苷酸重复微卫星位点，共76个多碱基重复微卫星位点。所有位点对浙江丽水学院两栖爬行动物实验室人工饲养的种群(n=30)进行了扩增和基因型分析。[结果]每个位点观察到的等位基因数和多态信息含量(PIC)的平均值分别为4个(2～7个)和0.514(0.164～0.822),观测杂合度(H_O)和期望杂合度(H_E)分别为0.00～0.97和0.18～0.82。有2个位点(THC141和THC377)显著偏离哈迪温伯格平衡，在11个位点中发现了显著的连锁不平衡。[结论]这些新筛选的多态性微卫星标记有助于进一步研究中国虎纹蛙的遗传多样性和开展遗传育种，为制定有效的保护策略和开展分子辅助选择提供依据。更多还原

【Abstract】 [Objective] Screening of high-quality polymorphism microsatellite markers in the Chinese tiger frog Hoplobatrachus chinensis can be used for identification, genetic diversity, population structure, and genetic linkage map analysis of this species. [Method] In this study, we identified 76 multiple-base repeat microsatellite loci, including 33 trinucleotide, 42 tetranucleotide and 1 pentanucleotide repeat microsatellite loci from partial genome of Chinese tiger frog Hoplobatrachus chinensis using the next-generation sequencing technology. We genotyped 30 individuals from a population in an artificial pond at the herpetological laboratory of Lishui University, Zhejiang Province, China. [Result] The results showed that the mean number of observed alleles and polymorphic information content(PIC) per locus was 4(range from 2 to 7) and 0.514(range from 0.164 to 0.822), respectively. The observed and expected heterozygosity values ranged from 0.00 to 0.97 and from 0.18 to 0.82, respectively. Two loci(THC141 and THC377) showed significant departure from Hardy-Weinberg equilibrium and significant linkage disequilibrium was found among 11 pairwise loci. [Conclusion] These novel polymorphic microsatellite markers are expected to be valuable for future studies on genetic diversity and conducting genetic breeding, enabling the formulation of effective conservation strategies and the use of molecular assisted selection for H. chinensis.更多还原