【摘要】 为了检测广西多棘蜈蚣毒腺转录组文库新基因CaTX-Ssmd1编码的多肽是否具有阻断钙离子通道蛋白的生物活性，将构建的pGEX-4T-1-CaTX-Ssmd1原核表达载体转化Escherichia coli Rosetta（DE3），诱导表达产物经酶切和RP-HPLC分离后得到CaTX-Ssmd1成熟多肽.质谱检测显示CaTX-Ssmd1多肽分子量与理论值一致；钙成像实验表明CaTX-Ssmd1多肽能有效减弱高钾外液灌流激发的小鼠背根神经节（dorsal root ganglion,DRG）细胞[Ca²⁺]_i信号.结论：初步验证了原核表达纯化的CaTX-Ssmd1多肽为新发现的电压门控性钙通道（voltage gated calcium channels,VGCC）阻断剂，丰富了多棘蜈蚣生物活性多肽资源库.更多还原

【Abstract】 To test whether the polypeptide encoded by the new gene CaTX-Ssmd1 in the venom gland transcriptome library of Scolopendra subspinipes multidens has the activity to block calcium channel protein,the prokaryotic expression vector pGEX-4T-1-CaTX-Ssmd1 was transformed into Escherichia coli Rosetta （DE3）.The mature CaTX-Ssmd1polypeptide was obtained from the induced expression product after digestion and RP-HPLC separation.Mass spectrometry analysis showed that the molecular weight of CaTX-Ssmd1 polypeptide was consistent with the theoretical value.Calcium imaging experiment shows that CaTX-Ssmd1 polypeptide can effectively attenuate[Ca²⁺]_i signal of mouse dorsal root ganglion （DRG） cells stimulated by high potassium external liquid perfusion.Conclusions:This study preliminarily verifies that the prokaryotic expression and purification of CaTX-Ssmd1 polypeptide is a newly discovered voltage gated calcium channels （VGCC） blocker,which enriches the resource pool of bioactive polypeptides of Scolopendra subspinipes multidens.更多还原