【摘要】 为筛选抗牛传染性鼻气管炎病毒(IBRV)的中药，本研究选取大青叶、金银花、鱼腥草、连翘和黄连，测定其对MDBK细胞的最大安全浓度，在此基础上通过计算各药物对病毒的抑制率分析上述中药对IBRV的直接杀伤作用、对IBRV黏附细胞的阻断作用和对IBRV在细胞内的抑制作用，确定上述中药对IBRV的有效抑制率；进一步，利用q PCR方法检测中药与IBRV作用后对MDBK细胞中细胞因子转录水平的影响。结果显示，连翘、金银花、黄连、大青叶和鱼腥草对MDBK细胞的最大药物安全浓度分别是3.125 mg/mL、3.125 mg/mL、0.780 mg/mL、1.560 mg/mL和0.780 mg/m L；大青叶对IBRV的直接杀伤作用最好，金银花对IBRV黏附细胞的阻断作用和对细胞内IBRV复制的抑制作用最好；鱼腥草可显著提高细胞中TNF-α、IL-2和IFN-γ的转录水平(P<0.001)；大青叶可极显著提高细胞中IL-1β、IL-2、IL-4、TNF-α和IFN-γ的转录水平(P<0.001)；黄连可极显著提高细胞中IL-1β的转录水平(P<0.001)。结果表明连翘、金银花、黄连、大青叶和鱼腥草均有抗IBRV作用，其抗病毒方式并不相同，本研究为抗IBRV中药的研发奠定基础。更多还原

【Abstract】 In order to analyze the anti-bovine infectious rhinotracheitis virus(IBRV) effect of the aqueous extract of Chinese herbal medicine, five Chinese herbal medicines, Da Qing Ye, Jin Yin Hua, Yu Xing Cao, Lian Qiao and Huang Lian, were selected to determine the maximum safe concentration on MDBK cells, the direct killing effect of these drugs on IBRV, the blocking effect on IBRV adhesion cells and the inhibiting effect on IBRV replication. Cell viability was detected by CCK-8 kit,and the effective inhibition rate of different concentrations of drugs on IBRV was obtained. Further, the effect of Chinese medicine and IBRV on the transcription level of cytokines after acting in MDBK cells was analyzed by qPCR method. The results showed that the maximum drug safe concentrations of Lian Qiao, Jin Yin Hua, Huang Lian, Da Qing Ye and Yu Xing Cao were3.125mg/mL, 3.125mg/mL, 0.780mg/mL, 1.560mg/mL and 0.780mg/mL on MDBK cells, respectively. Da Qing Ye had the best direct killing effect on IBRV, Jin Yin Hua had the best blocking effect on IBRV adhesion cells and the best inhibiting effect on IBRV replication; Yu Xing Cao significantly increased the transcription level of cellular TNF-α, IL-2 and IFN-γ(P<0.001); Da Qing Ye significantly increased the transcription level of cellular IL-1β, IL-2, IL-4, TNF-α and IFN-γ(P<0.001); Huang Lian significantly increased the transcription level of cellular IL-1β(P<0.001), these cytokines play important roles in the antiviral effect.The results showed that all the five herbal medicines above were safe for MDBK cells, and that Lian Qiao, Jin Yin Hua, Huang Lian, Da Qing Ye and Yu Xing Cao had different degrees of anti-IBRV effects, which laid a foundation for the development of anti-IBRV herbal medicines.更多还原