【摘要】 目的：探讨柠檬醛（citral）对小鼠B细胞淋巴瘤38B9细胞的作用和机制。方法：采用不同质量浓度（0～120 mg/L）的柠檬醛处理38B9细胞，通过CCK-8法检测细胞活力，并计算柠檬醛的半数抑制浓度（IC50）。根据IC50值设置低、中、高三个浓度（5、10、20 mg/L）处理38B9细胞，并检测柠檬醛对38B9细胞增殖的影响。采用瑞氏-吉姆萨染色观察38B9细胞形态变化。通过流式细胞术检测38B9细胞凋亡及细胞周期。利用RT-qPCR和Western blot检测38B9细胞凋亡及细胞周期相关因子的mRNA和蛋白表达水平，小鼠抑瘤实验观察柠檬醛对38B9细胞生长的作用。通过PubChem在线预测柠檬醛可能的靶点，并绘制蛋白质互作网络图。使用ClusterProfile对结果进行GO富集分析并使用ggplot2绘制可视化柱状图。利用Western blot检测38B9细胞MDM2/p53信号通路相关蛋白的表达水平。结果：柠檬醛能有效抑制小鼠B细胞淋巴瘤38B9细胞的活力，诱导其凋亡并阻滞其细胞周期，促进Puma、Noxa、Bax和p21的mRNA和蛋白表达（P<0.05或P<0.01），抑制Bcl-2和CDK2的mRNA和蛋白表达（P<0.05或P<0.01），抑制MDM2的蛋白表达（P<0.05），促进p53的蛋白表达（P<0.05）。结论：柠檬醛能够显著抑制小鼠B细胞淋巴瘤38B9细胞的增殖，同时诱导细胞凋亡并引起细胞周期阻滞，其机制可能与MDM2/p53信号通路有关。更多还原

【Abstract】 AIM: To explore the effect of citral on mouse B-cell lymphoma 38B9 cell line and its mechanism.METHODS: The 38B9 cells were treated with citral at different concentrations(0～120 mg/L). The cell viability was measured by CCK-8 assay, and then half-maximal inhibitory concentration(IC50) of citral was caculated. Wright-Giemsa staining was used to observed the morphological changes of 38B9 cells. Flow cytometry was used to analyze the apoptosis and cell cycle. RT-qPCR and Western blot were used to determine the mRNA and protein expression levels of apoptosisand cell cycle-related factors. The anti-tumor experiment in mice was performed to confirm the anti-cancer effect of citral on the growth of 38B9 cells. The possible targets of citral were predicted by PubChem, and the protein-protein interaction networks were drawn by Cytoscape. The GO enrichment analysis for the results was performed by ClusterProfile, and the bar chart was drawn through ggplot2. Western blot was used to determine the expression levels of MDM2/p53 signaling pathway-related proteins. RESULTS: Citral effectively inhibited the viability of mouse B-cell lymphoma 38B9 cells, and induced cell apoptosis and cell cycle arrest. Citral promoted the mRNA and protein expression of Puma, Noxa, Bax and p21( P<0. 05 or P<0. 01), inhibited the mRNA and protein expression of Bcl-2 and CDK2( P<0. 05 or P<0. 01), inhibited the protein expression of MDM2(P<0. 05), and promoted the protein expression of p53(P<0. 05). CONCLUSION:Citral can significantly inhibit the proliferation of mouse B-cell lymphoma 38B9 cells, and induce cell apoptosis and cell cycle arrest. The mechanism may be related to the MDM2/p53 signaling pathway.更多还原