【摘要】 为解决无患子组织培养中的难题，对愈伤组织诱导最佳外植体与基本培养基类型先进行筛选，再通过正交试验设计，筛选愈伤组织诱导植物生长调节剂、生长及分化培养最佳抗褐化剂和植物生长调节剂种类与浓度水平。结果显示：(1)1 a生嫩茎段是无患子愈伤组织诱导最佳外植体；(2)培养基中添加20.0 mg/L抗坏血酸和10.0 mg/L半胱氨酸能有效抑制愈伤组织褐化现象的发生；(3)以改良MS作为基本培养基，外加6-苄基腺嘌呤(6-BA)0.6 mg/L+糠基腺嘌呤(KT)0.15 mg/L+2,4-二氯苯氧乙酸(2,4-D)3.0 mg/L植物生长调节剂浓度组合，愈伤组织诱导率最高，结构致密；(4)愈伤组织团在改良MS外加6-BA 3.0 mg/L+萘乙酸(NAA)0.4 mg/L+吲哚丁酸(IBA)0.25 mg/L植物生长调节剂浓度组合下，不定芽诱导率最高，芽苗健壮。更多还原

【Abstract】 In order to solve the problem of tissue culture of Sapindus mukorossi Gaertn, the best explants and basic medium types for callus induction were screened first, and the types and concentration levels of plant growth regulators for callus induction, growth and differentiation culture were screened through orthogonal test design.The results showed that:(1)The annual tender stem segment was the best explant for callus induction of S.mukorossi Gaertn;(2)Addition of 20.0 mg/L ascorbic acid and 10 mg/L cysteine to the medium could effectively inhibit the occurrence of callus browning;(3)With modified MS as the basic medium and 6-BA 0.6 mg/L+KT 0.15 mg/L+2,4-D 3.0 mg/L plant growth regulator concentration combination, the callus induction rate was the highest and the structure was dense;(4)Under the combination of modified MS plus 6-BA 3.0 mg/L+NAA 0.4 mg/L+IBA 0.25 mg/L plant growth regulator concentration, the rate of adventitious bud induction was the highest and the bud seedlings were robust.更多还原