【摘要】 该研究对常规本科细胞生物学实验“细胞凝集反应”实验进行综合性扩展,在“细胞凝集反应”实验中融入糖抑制实验,帮助学生充分理解和掌握凝集素特性及细胞表面糖基成分;通过改良实验方法,用0.4%锥虫蓝对实验中的细胞进行染色后再用倒置显微镜观察,可以准确判断细胞凝集的起始浓度;优化凝集程度判断方法,采用细胞连接与细胞分散程度比例两个指标来共同判定细胞凝集程度,可更准确分析实验结果;通过改进授课方式,将社会热点问题和思政元素引人课堂,学生自主学习兴趣得到充分提升。该实验经以上4个方面改进探究,学生能更直观观察到在凝集素作用下细胞间的黏连现象,在合理准确分析实验结果的基础上加深理解凝集素对糖位点的特异性结合和细胞质膜上糖基的种类及功能,培养学生独立发现问题及分析解决问题的能力,提升实验教学效果。0.4%锥虫蓝染色镜检法和细胞凝集程度判断法为今后本科实验教学和科研工作者直观观察细胞凝集现象及差异性提供更便捷的方法,也为精准判断凝集素在低浓度下对细胞凝集的起始浓度界定提供可循依据和创新方向。更多还原

【Abstract】 In this study, the conventional undergraduate cell biology experiment “cell agglutination reaction” experiment was comprehensively expanded, and sugar inhibition experiment was incorporated into the “cell agglutination reaction” experiment to help students fully understand and master the characteristics of lectin and the glycobase components on the cell surface. By improving the experimental method, 0.4% Trypan blue was used to stain the cells in the experiment and then observed with an inverted microscope, the initial concentration of cell agglutination could be accurately determined. the method of judging the degree of agglutination was optimized, and the proportion of cell junction and cell dispersion was used to determine the degree of cell agglutination, which could analyze the experimental results more accurately. By improving the teaching method, the hot social issues and ideological and political elements can be organically introduced into the classroom, and the students’ interest in independent learning can be fully enhanced. Through the improvement and exploration of the above four aspects,students can more directly observe the adhesion phenomenon between cells under the action of lectins, and further understand the specific binding of lectins to sugar sites and the types and functions of sugar groups on the cell plasma membrane on the basis of reasonable and accurate analysis of the experimental results. Cultivate students’ ability to discover and solve problems independently, and improve the experimental teaching effect. 0.4%Trypan Blue staining microscopy method and cell agglutination degree judgment method provide a more convenient method for undergraduate experimental teaching and research workers to visually observe the phenomenon and difference of cell agglutination in the future. and also provide a basis and innovative direction for accurately judging the definition of initial concentration of cell agglutination under low concentration of lectin, and also provide a basis and innovative direction for accurately judging the definition of initial concentration of cell agglutination under low concentration of lectin.更多还原