【摘要】 目的 探讨B(A)血型患者的血清学及其基因型鉴定分析方法。方法 应用试管法(血清学)对3名患者的临床疑难ABO血型标本做ABO血型正反定型；应用实时荧光定量PCR技术对3例标本做ABO血型基因分型，并采用基因测序方法对其ABO基因1-7外显子测序确定基因型。结果 3名患者血清学ABO血型正反定型均表现为“B(A)血型”的格局；3例ABO基因分型均为B/O2,测序均在第7外显子B等位基因基础上发生c.640A>G突变，符合ABO~*BA.04基因型特点。结论 对于ABO血型亚型等疑难血型的鉴定，采用血清学和基因检测相结合的方法可以准确鉴定血型，保证临床用血安全。更多还原

【Abstract】 Objective To investigate the serology and genotype identification method of B(A) subtype patients. Methods Test tube method(serology) was used to confirm the clinically difficult ABO blood group samples of 3 patients with ABO blood group; ABO blood group was genotyped by real-time PCR, and the ABO gene exon 1-7 was sequenced to determine the genotype. Results The forward and reverse blood typing result of three patients was B(A) subtype all with ABO genotype B/O2 and c.640A> G mutation on B allele of exon 7, which meets the characteristics of ABO~*BA.04 genotype. Conclusion The combination of serological and genetic testing could identify difficult blood types such as ABO subtypes accurately and ensure the safety of clinical blood use.更多还原