【摘要】 [目的]探究草地贪夜蛾诱发人畜危害的分子机制，以草地贪夜蛾诱发人畜呼吸道炎症、疼痛等危害的潜在生理靶点(瞬时电位感受器香草酸受体1,TRPV1)为切入点，揭示草地贪夜蛾体表物质诱发人畜危害的毒素物质基础和生理靶点。[方法]通过凝胶分子筛以及高效液相色谱等方法对草地贪夜蛾体表物质进行分离和纯化，结合膜片钳电生理技术筛选草地贪夜蛾体表物质中靶向TRPV1的活性毒素组分。[结果]从草地贪夜蛾体表物质中分离纯化到一种能够高效(100 mg/L)激活TRPV1的毒素组分V-7,该组分能特异性作用于TRPV1,而对常见离子通道类膜蛋白生理受体(如钠离子通道NaV1.4、NaV1.5、NaV1.7,以及钾离子通道KV1.1、KV2.1、KV4.1)没有作用。[结论]从草地贪夜蛾体表混合物中可分离得到一种高效且特异性激活动物生理靶点TRPV1的毒素组分，部分揭示了外来入侵农业害虫草地贪夜蛾诱发人畜危害的物质基础及生理靶点。更多还原

【Abstract】 [Objective]In order to explore the molecular mechanism of human-animal hazards induced by the fall armyworm, the potential physiological targets(transient receptor potential vanillic acid subtype 1, TRPV1) inducing human and animal respiratory inflammation and pain were used as the entry point, and the toxin material basis and physiological targets of the body surface substance of Spodoptera frugiperda were used as the entry point to reveal the toxin substance basis and physiological target of the human and animal hazards induced by the material on the surface of Spodoptera frugiperda. [Method]The body surface substance of the grass moth was isolated and purified by gel molecular sieve and high performance liquid chromatography, and the active toxin components targeting TRPV1 in the body surface sunstance of the grass night moth were screened by combining patch clamp electrophysiology technology. [Result]A toxin component V-7 that can efficiently(100 mg/L) activate TRPV1 was isolated and purified from the body surface substance of the grassland nightcrawler, which can act specifically on TRPV1, but has no effect on common ion channel membrane protein physiological receptors(such as sodium channel Nav1.4, Nav1.5, Nav1.7, and potassium channel Kv1.1, Kv2.1, Kv4.1). [Conclusion]It is shown that a toxin component that efficiently and specifically activates the animal physiological target TRPV1 can be isolated from the body surface substance of Spodoptera frugiperda, which partially reveals the material basis and physiological target of the human and animal hazards induced by the invasive invasion of Spodoptera frugiperda in agriculture and forestry.更多还原