【摘要】 目的 构建中医寒湿痹证小鼠模型，探索“寒淫”“湿淫”致痹的可能机制。方法 成功构建胶原诱导型关节炎(Collagen induced arthritis,CIA)小鼠模型后，在不同温度、湿度下制备中医寒湿痹证动物模型，通过观察各组模型小鼠的舌苔、体质量、足踝肿胀程度、组织病理变化，ELISA检测血清中TNF-α的表达情况，探讨“寒淫”“湿淫”致病的免疫机制。结果 造模成功后，予寒湿两邪干预25 d后，与正常对照组相比，模型组、降温低湿组、降温高湿组、低温低湿组及低温高湿组寒湿痹证小鼠舌苔颜色黑紫；足踝关节明显肿大，组织切片可见淋巴细胞浸润明显；外周血中TNF-α均明显上升（P <0.05）。结论CIA小鼠血清中的TNF-α增高是中医六淫中“寒淫”“湿淫”造成踝关节痹证的主要原因之一；温度在7～10℃，湿度在80%～100%的条件可用于制备中医寒湿痹证小鼠动物模型。更多还原

【Abstract】 Objective To establish the mouse model of cold-dampness arthromyodynia and research on the mechanism of arthromyodynia induced by cold and dampness. Methods After the successful construction of CIA mouse model, the animal model of cold-dampness arthromyodynia in traditional Chinese medicine was established on the condition with different temperature and humidity. By observing the tongue coating, body weight, ankle swelling and histomathological changes, the level of TNF-α in serum, we explored the immune regulation mechanism of the arthromyodynia induced by cold and dampness. Results After CIA mouse model had been established for 14 days, we gave the stimulation to the CIA mouse model on different condition with cold and dampness. Compared with the normal control group, the model group stimulated with cold and dampness had black and purple tongue, obvious swollen ankle joint, its joint tissue was showed obvious lymphocytes infiltration, TNF-α in serum was significantly increased(P<0.05). Conclusion Cold and dampness can cause the arthralgia in the ankle joint of the mouse model of cold-dampness arthromyodynia by increasing the level of TNF-α in serum. Temperature at 7～10℃ and humidity at 80%～100% should be used as a modeling condition of the mouse model of cold-dampness arthromyodynia in triditional Chinese medicine.更多还原