【摘要】 基于斑马鱼胚胎行为学建立斑马鱼刺激模型，分别采用该模型和传统斑马鱼炎症模型对甘草酸二钾和黄芩素的舒缓功效进行验证对比；利用斑马鱼刺激模型筛选评价α-红没药醇、丹皮酚、积雪草苷、原花青素和4-叔丁基环己醇5种化妆品原料的舒缓效果。结果表明：与4-丁基间苯二酚相比，十二烷基硫酸钠(SDS)是稳定性更好的造模剂；以SDS浓度500μmol/L、对斑马鱼胚胎作用时间15 min构建的斑马鱼刺激模型评价甘草酸二钾和黄芩素的舒缓功效，结果与斑马鱼炎症模型基本一致；在高浓度下，α-红没药醇、丹皮酚、积雪草苷、原花青素在斑马鱼刺激模型中表现出较好的舒缓效果。更多还原

【Abstract】 In this research, a zebrafish stimulation model was established based on zebrafish embryonic behavior. The soothing effects of dipotassium glycyrrhizinate and baicalein were verified and compared with the traditional zebrafish inflammation model. Then, the soothing efficacy of five cosmetic raw materials including α-red myrrh alcohol, paeonol, asiaticoside, proantho cyanidins and 4-tert-butylcyclohexanol was selectively evaluated by this model. The results showed that compared with 4-butyl-resorcinol, sodium dodecyl sulfate was a more stable modeling agent. The zebrafish stimulation model constructed by SDS concentration of 500 μmol/L and 15 min on zebrafish embryos was used to evaluate the soothing effects of dipotassium glycyrrhizinate and baicalein, and the results were basically consistent with the zebrafish inflammation model. At high concentrations, α-red myrrh alcohol, paeonol, asiaticoside and proantho cyanidins showed significantly soothing efficacy in the zebrafish stimulation model.更多还原