【摘要】 通过对比不同培养料配方对桑黄（Inonotus hispidus）菌丝萌发、子实体生长及多糖、黄酮类、三萜类活性成分含量的影响，筛选出利用莲子壳人工栽培桑黄的培养料配方，同时开展桑黄子实体不同溶剂提取物对大肠杆菌、金黄色葡萄球菌以及白色念珠菌的抑菌效果研究。结果表明，木屑、木屑棉籽壳和麦粒培养料均可用于桑黄原种培养；相同培养条件下，莲子壳粉40%、木屑30%、麦麸16%、玉米粉10%、葡萄糖2%、石灰1%、石膏1%的配方为最适栽培培养料，此条件下桑黄菌丝生长最快，产量最高，并且子实体粗多糖、黄酮类含量最高。对该配方栽培的桑黄子实体开展了抑菌效果试验，4种不同溶剂提取物对大肠杆菌、金黄色葡萄球菌以及白色念珠菌3种指示菌都具有一定抑制效果，不同溶剂提取物对不同指示菌的抑菌效果存在一定差异。更多还原

【Abstract】 Through comparing the effects of different substrate formulas on mycelial growth, fruit body growth and the crude polysaccharide, flavone and triterpenes content of Inonotus hispidus, the suitable substrate formula with lotus seed shell for artificial cultivation of I. hispidus was selected. To investigate the antibacterial effect of different solvent extracts from fruit body of I. hispidus on Bacillus coli, Staphylococcus aureus and Candida. The results showed that sawdust, sawdust and cotton seed shell and wheat grain substrate could be used for cultivating the morther spawn of I. hispidus. Under the same culture conditions,formula with 40% lotus seed shell powder, 30% sawdust, 16% wheat bran, 10% corn meal, 2% glucose, 1% lime and 1% gypsum was the most suitable. Under this condition, the I. hispidus hyphae growth rate was the fastest, the yield was the hightat,and the crude polysaccharide, flavone and triterpenes content of fruit body was the highest. The results showed that four different solvent extracts had certain inhibitory effect on Bacillus coli, Staphylococcus aureus and Candida, and different solvent extracts had certain inhibitory effect on different indicator bacteria.更多还原