【摘要】 目的 探讨聚乙烯(PE)微塑料、聚丙烯(PP)微塑料及二者联合应用对雄性小鼠生殖损伤效应，并初步探讨其机制.方法 根据2×2析因设计将32只C57BL/6雄性小鼠随机分为阴性对照组、PE微塑料染毒组、PP微塑料染毒组及PE+PP微塑料联合染毒组.对照组喂饲正常饲料，微塑料单独染毒组喂饲添加1%的PE或PP饲料，联合染毒组喂饲同时添加1%PE和1%PP的饲料.饲养60 d后分析各组小鼠精子质量、血清性激素水平及睾丸组织氧化应激情况.结果 与对照组比较，PE组、PP组、PE+PP组小鼠精子畸形率增加(P<0.05);PE+PP组精子数量、精子活力下降明显(P<0.05).与对照组比较，联合染毒组小鼠血清睾酮水平、黄体生成素(LH)水平明显减少(P<0.05),PE组、PP组、PE+PP组小鼠血清卵泡刺激素(FSH)明显上升(P<0.05).与对照组比较，PE组、PP组、PE+PP组小鼠睾丸组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性降低(P<0.05),膜脂过氧化产物MDA含量明显增高(P<0.05).析因分析表明：微塑料PE、微塑料PP在降低精子数量和精子活力、增加MDA含量、降低睾酮生成方面存在交互增强作用.结论 微塑料PE、微塑料PP对小鼠生殖的损伤具有协同作用，其机制与氧化损伤及干扰生殖内分泌功能有关.更多还原

【Abstract】 Objective To investigate the effects of polyethylene(PE) microplastics, polypropylene(PP) microplastics and their joint action on reproductive injury in male mice, and to explore the injury mechanism preliminarily.Method Thirty-two male C57BL/6 mice were randomly divided into negative control group, PE microplastic exposure group, PP microplastic exposure group, PE and PP microplastic combined exposure group.The mice in control group were fed with normal feed, the mice in microplastic exposure group were fed with 1% PE or 1% PP feed, and the mice in combined exposure group were fed with 1% PE and 1% PP feed.After feeding for 60 days, the sperm quality, serum sex hormone levels and oxidative stress index of testicular tissue were analyzed in each group mice.Results Compared with the control group, the sperm deformity rate of mice in PE group, PP group and PE+PP group increased(P<0.05);The sperm density and sperm viability in PE+PP group decreased significantly(P<0.05).Compared with the control group, the serum levels of testosterone and luteinizing hormone(LH) in combined exposure group significantly decreased(P<0.05),and the levels of serum follicle stimulating hormone(FSH) in PE group, PP group and PE+PP groups significantly increased(P<0.05).Compared with the control group, the activities of superoxide dismutase(SOD) and catalase(CAT) in testicular tissue of mice decreased(P<0.05)in PE group, PP group and PE+PP group, while the contents of MDA significantly increased(P<0.05) in these microplastics exposure groups.The result of factorial analysis showed that there were interactive enhancement effect between microplastics PE and microplastics PP on decreasing sperm count and sperm motility, increasing MDA content and decreasing testosterone production.Conclusion Microplastic PE combined with microplastic PP has a synergistic effect on the reproductive injury of mice, and its mechanism might be related to oxidative damage and disturbance of reproductive endocrine function.更多还原