【摘要】 为了建立更加快速高效的测定肉制品中核苷酸的方法，文章以6种5′-单核苷酸钠盐（肌苷-5′-单磷酸二钠（5′-IMP）、鸟苷-5′-单磷酸二钠（5′-GMP）、胞苷-5′-单磷酸二钠（5′-CMP）、腺苷-5′-单磷酸二钠（5′-AMP）、尿苷-5′-单磷酸二钠（5′-UMP）、黄苷-5′-单磷酸二钠（5′-XMP））为标准品，制备混合标准溶液，通过色谱条件优化，建立了可同时测定这6种5′-单核苷酸的HPLC方法。具体色谱条件：C₁₈色谱柱，以甲醇（2%～15%）和0.05 mol/L磷酸二氢钾溶液（98%～85%）为流动相进行梯度洗脱，紫外检测波长250 nm,柱温30℃,进样量10μL。在该检测条件下，6种5′-单核苷酸均能较好分离，线性相关系数均大于0.99。将该方法用于冷鲜调理猪肉进行验证，发现其中的6种5′-单核苷酸均能一次性检出，每种5′-单核苷酸的加标回收率在89%～126%之间，RSD值在0.04%～2.21%之间。方法学验证结果表明，该方法操作便捷，准确度高，重现性好，可以用于肉制品中核苷酸含量的测定。更多还原

【Abstract】 To establish a more rapid and efficient method for the determination of nucleotides in meat products, six kinds of 5’-mononucleotide sodium（inosine-5’-monophosphate disodium（5’-IMP）, guanosine-5’-monophosphate disodium（5’-GMP）, cytidine-5’-monophosphate disodium（5’-CMP）, adenosine-5’-monophosphate disodium（5’-AMP）,uridine-5’-monophosphate disodium（5’-UMP）,xanthosine-5’-monophosphate disodium（5’-XMP）） are used as the standard samples to prepare mixed standard solution. Through the optimization of the chromatographic conditions, a HPLC method for simultaneous determination of the six 5’-mononucleotides is established. The specific chromatographic conditions are as follows: C₁₈ chromatographic column, gradient elution with methanol（2%～15%） and 0.05 mol/L potassium dihydrogen phosphate solution（98%～85%） as the mobile phase, UV detection wavelength of 250 nm, column temperature of 30 ℃, sampling volume of 10 μL.Under these detection conditions, the six 5’-mononucleotides can be separated well, and the linear correlation coefficients are all greater than 0.99. The method is applied to chilled and prepared pork for verification, and it is found that the six 5’-mononucleotides can be successfully detected at one time, the recovery rate of the six 5’-mononucleotides is 89%～126%, and the RSD value is 0.04%～2.21%.The methodology verification results indicate that the method is easy to operate, has high accuracy and good reproducibility, and can be used for the determination of nucleotide content in meat products.更多还原