【摘要】 目的 研究去甲斑蝥素（norcantharidin, NCTD）对鼻咽癌CNE-2细胞增殖和凋亡的影响。方法 CNE-2细胞分为对照组和不同剂量NCTD组，分别用含有0、35、70、140μmol/L NCTD的DMEM高糖培养基培养。采用CCK-8法、克隆形成实验、流式细胞术分别检测CNE-2细胞的增殖速率、克隆形成能力和凋亡率。实时定量PCR法检测各组CNE-2细胞中Cyclin B的mRNA表达水平。结果 48 h时，NCTD对CNE-2细胞的半数抑制浓度（IC50）值为（89.07±3.702）μmol/L；与对照组比较，35、70、140μmol/L NCTD组细胞增殖、克隆能力均下降，差异有统计学意义（P <0.05），细胞周期阻滞在G2/M期，细胞中Cyclin B的表达水平降低，差异有统计学意义（P <0.05），细胞凋亡率升高，差异有统计学意义（P <0.05）。结论 NCTD能够诱导CNE-2细胞的凋亡并通过下调Cyclin B的表达抑制细胞的增殖和克隆能力。更多还原

【Abstract】 Objective: To study the effects of norcantharidin(NCTD) on proliferation and apoptosis of nasopharyngeal carcinoma(NPC) CNE-2 cells. Methods: CNE-2 cells were divided into control group and different doses of NCTD group, and cultured in DMEM high glucose medium containing 0, 35, 70, 140 μmol/L NCTD. The proliferation rate, clonogenesis ability and apoptosis rate of CNE-2 cells were determined by CCK-8 assay, clonogenesis assay and flow cytometry, respectively. Cyclin B mRNA expression level in CNE-2 cells was detected by real-time quantitative PCR. Results: At 48h, the IC50 value of NCTD on CNE-2 cells was(89. 07 ± 3. 702) μmol/L. Compared with the control group, 35, 70 and 140 μmol/L NCTD groups significantly decreased cell proliferation and clonal formation ability(P < 0. 05), cell cycle arrest in G2/M phase, Cyclin B expression significantly decreased(P < 0. 05), and the apoptosis rate was significantly increased((P < 0. 05). Conclusion: NCTD can induce the apoptosis of CNE-2 cells and inhibit the proliferation and clonogenesis of CNE-2 cells by down-regulating Cyclin B expression.更多还原