【摘要】 为全面地剖析不同海拔地区及采摘期对刺梨中挥发性物质的影响，采用顶空固相微萃取（head-space solid phase microextraction,HS-SPME）提取不同海拔地区（记为A、B、C）的不同采摘期（记为1、2、3）的刺梨汁中的挥发性成分，并结合气相色谱-质谱联用（gas chromatography-mass spectrometry,GC-MS）对其进行分析，运用主成分分析（principal component analysis,PCA）和偏最小二乘法判别分析（prtial least squares discriminant analysis,PLS-DA）评价刺梨挥发性物质与海拔、采摘期的关系。结果表明，3个海拔地区的3个采摘期的刺梨汁中共检测到89种挥发性成分，包括酯类39种、醇类12种、醛酮类16种、呋喃类5种、芳香族6种、萜烯类5种、酸类6种，3个海拔地区的刺梨在成熟过程中均以酯类、醇类、醛酮类的变化为主。刺梨在A地区发育成熟最快，比B、C地区的刺梨发育分别快14、27 d左右。并找出3个海拔地区刺梨在成熟过程中差异物质。更多还原

【Abstract】 In order to comprehensively analyze the effects of different altitudes and picking periods on volatile compounds in Rosa roxburghii Tratt,head-space solid phase microextraction(HS-SPME) was used to extract volatile components from Rosa roxburghii Tratt at different altitudes(denoted as A, B and C) and different picking periods(denoted as 1,2 and 3),and analyze them by gas chromatography-mass spectrometry(GCMS). Principal component analysis(PCA) and partial least squares discriminant analysis(PLS-DA) were used to evaluate the relationship between altitude and picking periods of the volatile compounds of Rosa roxburghii Tratt. The results showed that a total of 89 volatile components were detected in Rosa roxburghii Tratt juice at three picking periods in three altitudes,including 39 esters,12 alcohols,16 aldehydes and ketones,5 furans,6 aromatics,5 terpenes and 6 acids. The changes of esters,alcohols,aldehydes and ketones in Rosa roxburghii Tratt were mainly observed during ripening at three altitudes. Rosa roxburghii Tratt matured fastest in A area,about 14 and 27 days faster than that in B area and C area, respectively, and found the main differential substances during the ripening process of Rosa roxburghii Tratt at three altitudes.更多还原