【摘要】 构建对邻苯二甲酸二（2-乙基）己酯（Bis（2-ethylhexyl） phthalate, DEHP）具有特异荧光响应的分子印迹-量子点传感器,应用于实际样品中DEHP的残留检测.采用改进的反相微乳液法,制备了对DEHP具有较高选择性和灵敏性响应的分子印迹-量子点纳米结构聚合物（MIP-QDs）,通过扫描电镜、红外光谱、X射线光电子能谱、选择性分析等方法对获得的MIP-QDs理化特性进行分析,成功将印迹层锚定在QDs上.进一步通过响应体系优化,结果表明,构建的MIP-QDs在V（水）:V（乙醇）=8:2、pH为8.0体系中,对DEHP具有较高的选择性响应;并在0.005～25.0 mg·L^-1的DEHP质量浓度范围内, DEHP质量浓度与荧光猝灭效率（F₀/F-1）间存在良好线性关系,相关系数为0.990 1,检测限低至1.5μg·L^-1,加标回收率为92.0%～96.8%,相对标准偏差低于8.2%;且在实际海水检测与GC-MS/MS检测结果之间具有良好的一致性.充分表明本文构建的MIP-QDs荧光传感器可用于海水中DEHP的实时定量检测.更多还原

【Abstract】 A molecularly imprinted polymer based on bis-（2-ethylhexyl） phthalate（DEHP） was prepared by an improved reverse micro emulsion method. A molecularly imprinted quantum dot fluorescence sensor（MIP-QDs）with specific fluorescence response to DEHP was constructed by embedding CdSe/ZnS quantum dots. The morphology of MIP-QDs was analyzed by SEM, FT-IR and X-ray photoelectron spectroscopy, and the detection system was optimized on this basis. The results showed that MIP-QDs had a high selective response to DEHP in V（water）:V（ethanol）=8:2 and pH 8.0 system, and had a good linear relationship with F₀/F-1 in the range of DEHP concentration from 0.005-25.0 mg·L^-1, with the correlation coefficient being 0.9901 and the detection limit being as low as 1.5 μg·L^-1. In actual seawater, the spiked recoveries of MIP-QDs ranged from 92.0%-96.8%, and the RSD was lower than 8.21%. There was no significant difference between MIP-QDs and GC-MS/MS in actual seawater detection, and the accuracy rate was not lower than 87.23%. In conclusion,MIP-QDs fluorescence sensor constructed in this paper can be used for real-time detection of DEHP in Marine water.更多还原