【摘要】 目的探讨肿瘤坏死因子受体相关蛋白1 (TRAP1)、连接蛋白43 (Cx43)在胶质瘤(GC)患者组织中的表达及预测术后复发的价值。方法 选取2018年4月至2019年4月新疆医科大学第二附属医院病理科94例GC患者术中标本为病理组,同期25例头部创伤脑减压术患者脑组织标本为正常组。逆转录聚合酶链式反应(RT-PCR)测TRAP1 mRNA、Cx43 mRNA表达。分析胶质瘤患者TRAP1 mRNA、Cx43 mRNA表达与病理特征的关系,分析GC复发因素,受试者工作特征曲线(ROC)分析TRAP1mRNA、Cx43 mRNA预测GC术后复发的价值。结果 病理组TRAP1 mRNA表达高于正常组,Cx43mRNA表达低于正常组(P <0.05);GC患者TRAP1 mRNA、Cx43 mRNA表达与病理分级、分化程度有关(P<0.05);将分化程度、病理分级控制后,TRAP1 mRNA高表达、Cx43 mRNA低表达均为GC患者术后复发的独立危险因素(P<0.05);ROC曲线显示,TRAP1 mRNA、Cx43 mRNA联合预测GC术后复发的AUC值为0.889,高于单独预测(P <0.05)。结论 GC患者组织中TRAP1 mRNA、Cx43 mRNA均异常表达,且与GC恶性进展密切相关,可为复发预测提供参考。更多还原

【Abstract】 Objective To explore the expression of tumor necrosis factor receptor related protein 1(TRAP1)and connexin 43(Cx43) in the tissues of patients with gliomatosis cerebral(GC) cerebral and the value of predicting postoperative recurrence.Methods The intraoperative specimens of 94 GC patients in our hospital from April 2018 to April 2019 were selected as the pathology group, and the brain tissue specimens of 25 patients with head trauma and decompression in the same period were selected as the normal group. The expression of TRAP 1 mRNA and Cx43 mRNA in brain tissue was detected by reverse transcription polymerase chain reaction(RT-PCR). The relationship between the expression of TRAP1 mRNA and Gx43 mRNA and pathological characteristics in patients with GC were analyzed. The risk factors for postoperative recurrence of GC patients were analyzed.The receiver operating characteristic curve(ROC) was used to analyze the value of TRAP1 mRNA and Cx43 mRNA in predicting postoperative recurrence of GC. Results The expression of TRAP1 mRNA in the observation group was higher than that of the control group, and the expression of Cx43 mRNA was lower than that of the control group(7,<0.05). The expression of TRAP1 mRNA and Cx43 mRNA in GC patients were related to pathological grade and degree of differentiation(P<0.05).After controlling the pathological grade and degree of differentiation, high expression of TRAP1 mRNA and low expression of Cx43 mRNA were independent risk factors for postoperative recurrence of GC patients(P<0.05). The ROC curve showed that the AUC value of the combination of TRAP1 mRNA and Cx43 mRNA for predicting postoperative recurrence of GC was 0.889, which was higher than the single prediction(P<0.05). Conclusion TRAP1 mRNA and Cx43 mRNA expressed abnormally in the tissues of patients with GC, and they were closely related to the malignant progression of GC, and can be used as indicators for predicting postoperative recurrence.更多还原