【摘要】 从酶活性和基因表达2个方面探讨了铜离子对中华绒螯蟹鳃ATP酶的影响。CuSO₄·5H₂O胁迫试验设置1个空白对照组和4个试验组，浓度分别为0、0.17、0.69、2.77和11.08 mg/L，于胁迫5和10 d取样，检测鳃ATP酶活性和相关基因表达情况。结果表明：随着铜离子浓度的增大，中华绒螯蟹鳃Na⁺/K⁺-ATP酶和Ca²⁺-ATP酶活性均呈现出先升高后降低的趋势，当浓度为0.69 mg/L时，2种酶的活性最大。同时，qPCR结果显示，NaK-ATPase、V-ATPase和NKCC基因表达量表现为先升高后降低的趋势，当浓度为0.69 mg/L时达到最大值；并且，铜胁迫10 d的ATP酶活性和基因表达水平比5 d的有所降低。这说明低浓度铜离子可以促进中华绒螯蟹鳃ATP酶活性，而高浓度铜离子则使ATP酶活性受到抑制；并且随着暴露时间的延长，ATP酶活性和基因表达水平有下降的趋势。更多还原

【Abstract】 The enzyme activity and gene expression were analyzed to discuss the effects of copper on gill ATPase of E.sinensis.A blank control group and 4 experimental groups of CuSO₄·5H₂O stress experiment were set up,which were 0,0.17,0.69,2.77 and 11.08 mg/L respectively.At 5 days and 10 days exposure,gill ATPase activity and related gene expression were detected.The results showed that with the increase of treatment concentrations,the Na⁺/K⁺-ATPase and Ca²⁺-ATPase activities of gill showed a trend of increasing first and then decreasing.The activities of the two enzymes reached peak at the copper concentration of 0.69 mg/L.The mRNA levels of NaK-ATPase,V-ATPase and NKCC showed a trend of increasing first and then decreasing,and reached the maximum at the concentration of 0.69 mg/L.The ATPase activities and gene expression levels at 10 days of copper stress were lower than those at 5 days.The study indicated that low concentration of copper ions could promote the gill ATPase activity of E.sinensis,while high concentration of copper ions could inhibit the ATPase activity.Moreover,with the prolongation of exposure time,ATPase activity and gene expression level showed a downward trend.更多还原