【摘要】 以“美酒”白掌顶芽为外植体，研究了白掌茎尖离体培养及组培快繁技术。结果表明：顶芽用0.6%含氯消毒溶液浸泡40 min后，再用0.1%HgCl2溶液消毒4 min，然后剥取直径1～2 mm茎尖，消毒成功率可达96%以上，茎尖全部存活。培养茎尖最适培养基为1/2MS+0.6 mg/L KT+0.1 mg/L IBA。增殖丛生芽最适培养基为3/4MS+1.5 mg/L 6-BA+0.1 mg/L IBA，培养40 d，增殖率为3.49；生根最适培养基为1/2MS+0.5 mg/L IBA,30 d生根率可达90.71%，在该培养基上生根的种苗移栽后的株高和生长量最大，35 d株高增加1.38 cm。更多还原

【Abstract】 The apical bud was used as explants to study the shoot-tip culture and rapid propagation of Spathiphyllum kochii ‘mojo’. The results showed that the apical buds of Spathiphyllum kochii ‘mojo’ were soaked in 0.6% chlorinated solution for 40min after 0.1% HgCl2 for 4 min, then the 1～2 mm shoot-tips in diameter were taken off. The disinfection rate of the shoot-tips was more than 96%, and all tips survived. The optimum medium for shoot-tip culture was the medium 1/2MS+0.6 mg/L KT+0.1mg/L IBA. The best medium for tufty bud proliferation was the medium 3/4MS+1.5 mg/L 6-BA+0.1 mg/L IBA. After 40 days of culture, the bud proliferation rate reached 3.49. The suitable rooting medium was the medium 1/2MS+0.5 mg/L IBA, and the rooting rate reached 90.71% in 30 days. The seedlings on this medium grew most after transplanting, which increased by 1.38 cm high in 35 days.更多还原