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基于HPLC的‘关山’樱花嫩红素成分——异樱花苷和樱花亭的定量分析
Quantitative analysis of isosakuranin, sakuranetin in Prunus lannesiana‘Alborosea’ based on HPLC
【摘要】 为了准确测定樱花中有关嫩红素主要成分的含量,该研究以我国南方主栽樱花品种‘关山’为试验材料,利用HPLC法对其花瓣干品内的嫩红素——异樱花苷和樱花亭进行定量分析。结果表明,在最适色谱条件下,异樱花苷的回归方程为Y=554.895 353X+1 567.125 008(R~2=0.999 809 96),樱花亭的回归方程为Y=221.578 696X+308.044 811(R~2=0.992 465 15)。试验的精密度、稳定性、重复性和加样回收率结果均为良好。最终样品进样得到‘关山’樱花干品中异樱花苷平均含量为539.97μg/g,但未检测到樱花亭含量。该研究结果为提高我国樱花资源的应用范围及我国樱花资源的开发、利用,提供一定的依据。
【Abstract】 To accurately determine the content of main components of emodin in cherry blossom, this study used Prunus lannesiana ‘Alborosea’ planted for main variety in South China as experimental material, isosakuranin and sakuranetin were quantitatively analyzed by HPLC. Results showed that under the optimum chromatographic conditions, the regression equation of isosakuranin was concluded as Y=554.895 353X+1 567.125 008(R~2=0.999 809 96), and that of sakuranetin as Y=221.578 696X+308.044 811(R~2=0.992 465 15). The precision, stability, repeatability and sample recovery of the experiment were satisfactory. The average content of isosakuranin was determined as 539.97 μg/g in P. lannesiana ‘Alborosea’ dry petal product but sakuranetin was not detected. This results could improve the application scope of cherry resources and provide a basis for the development and utilization of cherry resources in China.
【Key words】 Prunus lannesiana‘Alborosea’; Emodin; Isosakuranin; Sakuranetin; HPLC;
- 【文献出处】 江苏林业科技 ,Journal of Jiangsu Forestry Science & Technology , 编辑部邮箱 ,2022年01期
- 【分类号】R284.1
- 【下载频次】111