【摘要】 γ-氨基丁酸转氨酶(γ-aminobutyric acid transaminase GABA-T,EC2.6.1.19)是植物GABA转化途径中的第一步关键酶，与γ-氨基丁酸GABA的积累具有一定的关系.文中将苦荞进行发芽处理提取其总RNA,利用反转录聚合酶链式反应(reverse transcription polymerase chain reaction, RT-PCR)、巢氏PCR(nested PCR)与3′-cDNA末端快速扩增技术(rapid amplification of cDNA end, RACE)成功获得发芽苦荞GABA-T全长基因.序列分析表明，该基因序列全长1 556 bp,含有一个编码448个氨基酸的开放阅读框(open reading frame, ORF),其氨基酸序列与菠菜全长GABA-T氨基酸序列和欧洲栓皮栎树全长GABA-T氨基酸序列的相似度分别为87.05%和86.83%.基因序列的进化树分析结果表明，发芽苦荞GABA-T基因与甜菜、藜麦和菠菜来源相关基因的亲缘性最近.更多还原

【Abstract】 GABA transaminase GABA-T(EC 2.6.1.19) is the first key enzyme in the plant GABA transformation pathway, and is considered to have a certain relationship with the accumulation of GABA. In this study, the total RNA was extracted from germinated tartary buckwheat and the GABA-T gene was successfully cloned based on reverse transcription polymerase chain reaction(RT-PCR), nested PCR and rapid amplification of cDNA 3 ′-end(RACE) technologies. Sequence analysis showed that it was 1 556 bp in length and contained an open reading frame(ORF) encoding 448 amino acids. Its amino acid sequence has 87.05% and 86.83% similarity to the full-length GABA-T amino acid sequence of Spinacia oleracea and Eucalyptus urophylla, respectively. Phylogenetic tree analysis showed that it has the closest genetic relationship with sugar beet, buckwheat and spinach.更多还原