【摘要】 为实现北乌头（Aconitum kusnezoffii）种子活力快速测定，通过4因素3水平正交试验，探究了四唑染色法（TTC）测定北乌头种子活力中各因素对染色效果的影响，并确定了最适染色条件。结果表明，在各因素对种子染色效果的影响中，染色时间>TTC浓度>浸种时间>染色温度；测定种子生活力的最适条件为种子浸泡12 h后，30℃下0.3%TTC染色液染色15 h。更多还原

【Abstract】 In order to realize the rapid determination of the seed vigor of Aconitum kusnezoffii,through the orthogonal test of 4 factors and 3 levels,the influence of each factor in the seed vigour of Aconitum kusnezoffii on the dyeing effect was explored,and the optimal dyeing conditions were determined. The results showed that the influence of various factors on the seed dyeing effect was ranked as dyeing time>TTC concentration>soaking time>dyeing temperature. The most suitable condition for determining seed viability is after seed soaking for 12 h,staining with 0.3% TTC dye solution at 30 ℃ for 15 h.更多还原