【摘要】 目的：研究钙调神经磷酸酶（CaN）在慢性心力衰竭（CHF）阳虚水泛证患者血清中的表达及与临床指标相关性，初步拟定该证候的诊断界值。方法：收集CHF阳虚水泛证患者及健康对照者各30例，ELISA检测血清中CaN、N末端B型脑肽前体（NT-proBNP）的含量。结果：CaN在CHF阳虚水泛证组患者血清的含量为（1 347.54±252.56）pg/mL，显著高于健康对照组（710.49±281.26）pg/mL(P<0.01)；且随NYHA心功能分级的增加，CHF阳虚水泛证患者血清中CaN含量不断升高（P<0.01）。CHF阳虚水泛证患者血清中CaN水平与NYHA心功能分级呈正相关性（r=0.933,P=0.000）；与NT-proBNP呈正相关性（r=0.692,P=0.000）；与LVEF呈负相关性（r=-0.789,P=0.000）。绘制ROC曲线初步拟定血清中CaN诊断CHF阳虚水泛证的界值为1 080.36 pg/mL，曲线下曲积为0.97。结论：CaN可反映CHF病情轻重程度，可为CHF阳虚水泛证患者提供客观化、量化的参考。更多还原

【Abstract】 Objective: To study the expression of CaN in the serum of patients with chronic heart failure(CHF) of yangdeficiency-water-overflowing syndrome and its correlation with clinical indicators. Preliminarily draw up the diagnostic threshold of this syndrome. Methods: A total of 30 patients with CHF of yang-deficiency-water-overflowing syndrome and 30 healthy controls were collected. The content of CaN and NT-proBNP in serum were determined by ELISA. Results: The content of CaN in the serum of CHF patients with yang-deficiency-water-overflowing syndrome was(1 347.54±252.56) pg/mL, significantly higher than the healthy control group(710.49±281.26) pg/mL(P<0.01). Moreover, the content of CaN increased with the increase of cardiac function classification(P<0.01). In patients with CHF of yang-deficiency-water-overflowing syndrome, there was a positive correlation between CaN with cardiac function classification(r=0.933, P=0.000), there was positively correlated with NT-proBNP(r=0.692, P=0.000), there was negatively correlated with LVEF(r=-0.789, P=0.000). With ROC, preliminarily established the diagnostic threshold of CaN in yang-deficiency-water-overflowing syndrome of CHF was 1 080.36 pg/mL, the AUC was 0.97. Conclusion: CaN can reflect the severity of CHF and provide an objective and quantitative reference for yangdeficiency-water-overflowing syndrome of CHF.更多还原