【摘要】 本文利用H2O2脱色、酶法和Sevage法联合脱蛋白对野巴子粗多糖进行初步纯化并得到初步纯化多糖(YBZp),对YBZp采用Superdex-75和Superdex-30串联凝胶柱进行进一步纯化,得到野巴子多糖(YBZb)。经高效凝胶渗透色谱法检测为均一的纯化多糖,分子量6 400.3 u。YBZb的结构鉴定采用甲基化、气相色谱、气相色谱-质谱、核磁共振、红外光谱方法。结果表明,YBZb的单糖组成为阿拉伯糖、甘露糖、葡萄糖、半乳糖,相对摩尔比为0.526:0.061:0.166:0.247,YBZb是由4种单糖组成的中性杂多糖,其中,阿拉伯糖所占比例最高。YBZb具有多分枝结构,主链为1→6连接的半乳糖,在4位有分枝,且每16个半乳糖有11个分枝;支链由阿拉伯糖以1→5连接,葡萄糖以1→4连接构成,末端由阿拉伯糖、甘露糖、葡萄糖构成,存在α、β糖苷键。YBZb的活性实验表明,在浓度达到4 mg/m L时,YBZb对·OH、DPPH·的清除率最大分别为47.14%、90.62%,表明YBZb具有一定的清除·OH和DPPH·的活性。更多还原

【Abstract】 In this paper,the crude polysaccharide of Elsholtzia rugulosa H. was purified by H₂ O₂ decolorization,enzymatic method and Sevage method combined with deproteinization. The preliminary purified polysaccharide（ YBZp） was obtained. The YBZp was further purified by Superdex-75 and Superdex-30 tandem gel column to obtain YBZb. The purified polysaccharide with a molecular weight of 6400. 3 u. YbZb was identified by methylation,gas chromatography,gas chromatographymass spectrometry,nuclear magnetic resonance（ NMR） and infrared spectroscopy. The results showed that the monosaccharide composition of YBZb was Ara,Man,Glu and Gal,and the relative molar ratio was 0. 526: 0. 061: 0. 166: 0. 247. YBZb was a neutral heteropoly sugar composed of four monosaccharides,among which Ara accounted for the highest proportion. YBZb has a multibranched structure,the main chain is 1→6 linked Gal,branched at 4 sites,and every 16 Gal has11 branches. The branched chain is composed of Ara connected by 1→5 and Glu connected by 1→4. The end of the branched chain is composed of Ara,Man and Glu,and there are α and β glycosidic bonds. The results showed that the maximum scavenging rates of YBZb on ·OH and DPPH·were respectively 47. 14% and 90. 62%. when the concentration of YBZb reached 4 mg/m L,which indicated that YBZb had certain scavenging activity against ·OH and DPPH·.更多还原