【摘要】 为有效控制蒿板青颗粒的质量,采用高效液相色谱法对蒿板青颗粒中有效成分一枝蒿酮酸和（R, S）-告依春含量进行了研究。采用C₁₈柱（250 mm×4.6 mm, 5μm）,流动相为乙腈（A）-0.4%磷酸水溶液（B）,梯度洗脱（0 min, 5%A;0～10 min, 5%A→15%A;10～25 min, 15%A→30%A;25～40 min, 30%A;40～41 min, 30%A→5%A;41～45 min, 5%A）,检测波长为245 nm,流速1.0 mL/min,柱温35℃。结果表明,在该色谱条件下,辅料对一枝蒿酮酸和（R,S）-告依春出峰均无干扰,一枝蒿酮酸和（R, S）-告依春与相邻杂质峰分离良好;一枝蒿酮酸、（R, S）-告依春浓度分别在24～120μg/mL和4.8～24μg/mL范围内,峰面积与其对应的含量呈现良好的线性关系（一枝蒿酮酸R²=0.9992,3组样品中一枝蒿酮酸的平均回收率分别为98.4%、97.9%和98.1%,RSD分别为1.31%、1.02%和1.33%;（R, S）-告依春R²=0.9997,3组样品中（R, S）-告依春的平均回收率分别为98.8%、98.3%和97.5%,RSD分别为0.56%、0.88%和1.14%）。该方法简便、分析快速、准确度高,重复性好,适用于蒿板青颗粒中有效成分一枝蒿酮酸和（R, S）-告依春的质量控制。更多还原

【Abstract】 In order to improve the quality standard of Haobanqing granules, the contents of rupestonic acid and（R, S）-epigoitrin in Haobanqing granules was determined by HPLC. The isolation was performed on a C₁₈ column（4.6 mm × 250 mm, 5 μm） with mobile phase consisting of acetonitrile（A）–0.4% phosphoric acid aqueous solutions（B） using gradient elution（0 min, 5% A; 0～10 min, 5% A→15% A; 10～25 min, 15% A→30% A; 25～40 min, 30% A; 40～41 min, 30% A→5% A; 41～45 min, 5% A）. The detection wavelength was 245 nm, flow rat 1.0 mL/min, column tempterature 35 ℃. In the method, excipients and solvents did not Interfere with the peaks of rupestonic acid and（R, S）-epigoitrin; rupestonic acid and（R, S）-epigoitrin were well separated from adjacent impurity peaks. The calibration curves of rupestonic acid and（R, S）-epigoitrin were in good linearity within the ranges of 24～120 μg/mL（R²= 0.9992） and 4.8～24 μg/mL（R² = 0.9997）,（The average recovery of rupestonic acid in the three groups of samples were 98.4%, 97.9% and 98.1%, and the RSD were 1.31%,1.02% and 1.33%;The average recovery of（R, S）-epigoitrin in the three groups of samples were 98.8%, 98.3% and 97.5%, and the RSD were 0.56%,0.88% and 1.14%）. The results indicated that the method was simple, rapid, accurate and repeatable, and can used to control the content of rupestonic acid and（R, S）-epigoitrin in Haobanqing granules.更多还原