【摘要】 目的拟设计一种在无操作时能完全隔离感染动物,又能在日常照料动物时形成定向气流的实验装置,保证非人灵长类动物正常生物学特性表达的同时还能有效防止气溶胶感染。方法使用具有氯霉素抗性的大肠杆菌(Cl+,E.coli, Rosetta菌株)作为指示微生物,观察处于本实验装置中非人灵长类在实验中的生物学特性,并采用气溶胶采集装置及沉降菌采集的方法对本实验装置内外的指示微生物进行监测。结果在内笼指示菌富集时,本实验安全装置的外笼无论在开启还是关闭状态下,房间内、笼外及外笼均未检测到指示菌及气溶胶。结论本实验装置能保证非人灵长类正常生物学特性的表达,并可有效防止气溶胶感染。更多还原

【Abstract】 Few studies in China and abroad have examined the biosafety of experimental devices for Non-human primate, and the technology is not mature. Non-human primate are usually reared in single cages in an infectious environment. Although this type of cage can effectively isolate infected experimental animals, the environmental welfare of the Non-human primate population is not considered, and such housing is not suitable for long-term observations of infection experiments. In this study, a Non-human primate biosafety centralized feeding experimental device was designed, which not only completely isolates infected animals without operation but also provides directional airflow during the daily care of animals, thus both ensuring that the monkeys exhibit normal biological characteristics and effectively preventing aerosol infection. We observed the biological characteristics of Non-human primate in this experimental device. We then used chloramphenicol resistant Escherichia coli(Cl+, E.coli, Rosetta strain) as indicator microorganism, which was monitored inside and outside the experimental device with an aerosol collection device, according to the bacterial sedimentation collection method. When the indicator bacteria were enriched in the inner cage, the indicator bacteria and aerosol were not detected in the room or outside the cage, regardless of whether the outer cage of the safety device was open or closed. The experimental device not only ensures normal biological characteristics among Non-human primate but also effectively prevents aerosol infection.更多还原