【摘要】 目的探索五步蛇毒对正常小鼠免疫功能的影响。方法将KM小鼠随机分为5组,分别为空白组、五步蛇毒安全范围内低剂量组(30 mg/kg)、五步蛇毒安全范围内中剂量组(60 mg/kg)、五步蛇毒安全范围内高剂量组(120 mg/kg)(以下简称五步蛇毒低、中、高剂量组)以及西洋参组(30 mg/kg),每组10只。各组小鼠每日定时灌胃1次,连续14天。观察小鼠的呼吸、精神状态等生命体征。取小鼠脾脏和胸腺组织称重后,计算小鼠脾脏、胸腺指数;制备脾细胞悬液,采用CCK-8法检测小鼠脾淋巴细胞增殖率,碳粒廓清法检测小鼠吞噬系数α,中性红法检测小鼠腹腔巨噬细胞吞噬率;同时采用二硝基氟苯诱导小鼠迟发型变态反应,检测小鼠耳廓肿胀度。结果小鼠的吞噬系数α在五步蛇毒高剂量组最高(5.56±0.46),高于其余四组,差异均有统计学意义(均P<0.05)。小鼠腹腔巨噬细胞吞噬率(%)在五步蛇毒中剂量组和西洋参组中较高,分别为(119.07±19.31)%、(124.79±24.93)%,差异有统计学意义(均P<0.05)。小鼠的B淋巴细胞增殖率(%)在五步蛇毒中剂量组和西洋参组中较高,分别为(105.95±14.59)%、(108.14±10.58)%,差异有统计学意义(均P<0.05)。小鼠的T淋巴细胞增殖率(%)在五步蛇毒中、高剂量组中较高,分别为(119.30±20.07)%、(110.85±23.13)%,差异有统计学意义(均P<0.05)。与空白组比较,五步蛇毒中、高剂量组和西洋参组均能显著提高小鼠的耳廓肿胀度,差异有统计学意义(均P<0.05)。与空白组比较,五步蛇毒各剂量组和西洋参组均能显著提高小鼠脾脏指数(均P<0.05)。与空白组比较,各实验组胸腺指数均无明显差异(均P>0.05)。结论口服低于120 mg/kg的五步蛇毒对正常小鼠的免疫功能具有一定的增强作用。更多还原

【Abstract】 Objective To explore the effects of the Deinagkistrodon acutus venom on the immune function in normal mice. Methods The KM mice were randomly divided into 5 groups: blank group, low-dose group(30 mg/kg) within the safety range of Deinagkistrodon acutus venom, medium-dose group(60 mg/kg) within the safety range of Deinagkistrodon acutus venom, high-dose group(120 mg/kg) within the safety range of Deinagkistrodon acutus venom(hereinafter referred to as low, medium-and high-dose groups of Deinagkistrodon acutus venom) and American ginseng group(30 mg/kg),10 mice in each group.Gave the medicine once a day at regular intervals for 14 days.The vital signs such as respiration and mental state were observed.Took spleen and thymus tissues, calculate spleen and thymus index after weighing; prepared spleen cell suspension, used CCK-8 method to detect lymphocytes proliferation rate; carbon particle clearance method to detect mouse phagocytic coefficient α;the mouse peritoneal macrophage phagocytosis was detected by neutral red assay; dinitrofluorobenzene(DNFB) was used to induce delayed type hypersensitivity in mice and detected the auricle swelling of mice. Results The phagocytic coefficient α of mice in high-dose Agkistrodon acutus venom group was the highest(5.56±0.46),and the differences were statistically significant(all P<0.05).The phagocytosis rate(%) of mice were higher in the medium-dose group and the American ginseng group, which respectively were(119.07±19.31)% and(124.79±24.93)%,the differences were statistically significant(P<0.05).The B lymphocyte proliferation rate(%) of mice were higher in the medium-dose group of and the American ginseng group, which respectively were(105.95±14.59)% and(108.14±10.58)%,the differences were statistically significant(P<0.05).The T lymphocyte proliferation rate(%) of mice were higher in the medium-dose and high-dose groups, which respectively were(119.30±20.07)%and(110.85±23.13)%,the differences were statistically significant(P<0.05).Compared with the blank group, the medium-dose and high-dose groups and the American ginseng group could significantly improve the auricle swelling degree of mice, the differences were statistically significant(P<0.05).Compared with blank group, each experimental group could significantly increase the spleen index of mice(P<0.05).Compared with blank group, there was no significant difference in the thymus index of each experimental group(P>0.05). Conclusion Oral administration of the Deinagkistrodon acutus venom lower than 120 mg/kg has a certain enhancement effect on the immune function in normal mice.更多还原