【摘要】 目的利用转录组测序技术(RNA-seq)筛选与牙源性角化囊肿(OKC)增殖相关的关键基因。方法收集OKC囊壁组织及正常口腔黏膜组织标本并提取总RNA,利用RNA-seq分析全基因转录本的差异,并分析差异表达基因(DEGs)的功能。结果 OKC囊壁组织与正常口腔黏膜组织间共有359个DEGs,其中169个基因表达上调,190个基因表达下调。KEGG通路富集分析表明,注释在磷脂酰肌醇3-激酶/蛋白激酶B(PI3K-Akt)信号通路的DEGs共15个,包括8个上调基因和7个下调基因。结论 OKC中存在PI3K-Akt信号通路基因的异常表达,PI3K-Akt信号通路相关基因在OKC增殖中可能发挥一定的调控作用。更多还原

【Abstract】 Objective To investigate the key genes associated with the proliferation of odontogenic keratocyst(OKC) using the RNA-sequencing(RNA-seq) technique. Methods Total RNA was extracted from OKC cyst wall specimens and normal oral mucosal tissue specimens, and RNA-seq was used to analyze the difference in transcripts and the functions of differentially expressed genes(DEGs). Results A total of 359 DEGs were identified between OKC cyst wall specimens and normal oral mucosal tissue specimens, among which 169 were upregulated and 190 were downregulated. The KEGG pathway enrichment analysis showed that there were 15 DEGs annotated in the phosphoinositide 3-kinase(PI3 K)/protein kinase-B(Akt) signaling pathway, with 8 upregulated genes and 7 downregulated genes. Conclusion There is abnormal expression of PI3 K-Akt signaling pathway genes in OKC, and the PI3 K-Akt signaling pathway-related genes may play a certain regulatory role in the proliferation of OKC.更多还原