【摘要】 目的:建立同时测定人血浆中他莫昔芬、托瑞米芬及它们的代谢物的LC-MS/MS法。方法:使用岛津LCMS-8060型LC-MS/MS仪,以MRM模式测定他莫昔芬、托瑞米芬及它们的代谢物共6种成分,离子源为ESI源。色谱柱选用Waters ACQUITY BEH C₁₈（2.1×50mm,1.7μm）,梯度洗脱。血浆样本加入内标,经乙腈沉淀蛋白后取上清液进样检测。结果:所建方法经验证,其线性、准确度、精密度、最低定量限、提取回收率、特异性、基质效应、稳定性等各项指标均符合NMPA指导原则。结论:所建方法快速、灵敏,适用于同时测定人血浆中他莫昔芬、托瑞米芬及它们的代谢物。更多还原

【Abstract】 Objective:To establish a rapid and sensitive LC-MS/MS method for determination of tamoxifen,toremifene and their major active metabolites in human plasma.Method:Shimadzu LCMS-8060 series LC-MS/MS was used.6 components including tamoxifen,toremifene and their metabolites were monitored by ESI in MRM model.The analysts were eluted on Waters ACQUITY BEH C₁₈ column（2.1×50 mm,1.7μm） according to gradient elution program.The plasma samples were mixed with internal standard,then precipitated protein by acetonitrile,the supernatants were detected.Result:The linear,accuracy,precision,LLOQ,extraction recovery,specificity,matrix effect,stability of the method met the guiding principles and criteria of NMPA.Conclusion:The method is rapid,sensitive and suitable for the determination of tamoxifen,toremifene and their metabolites in human plasma.更多还原