【摘要】 为探讨Sin3A相关蛋白18 (SAP18)在宫颈癌组织中的表达情况,并研究SAP18对宫颈癌细胞HeLa增殖和迁移能力的影响与分子机制。利用TCGA和GTEx数据库分析SAP18在宫颈癌患者癌组织和癌旁组织中的表达水平,并计算SAP18表达水平与患者生存率的关系。免疫组织化学染色试验检测宫颈癌组织标本中SAP18蛋白的表达情况。CCK-8试验和软琼脂克隆形成试验检测SAP18对HeLa细胞增殖的影响,细胞划痕试验检测SAP18对HeLa细胞迁移能力的影响。免疫印迹法验证敲低人乳头瘤病毒(HPV)编码的E6、E7蛋白对SAP18表达水平的影响。结果表明:SAP18在宫颈癌组织中呈高表达;SAP18表达水平与宫颈癌患者生存率呈负相关。过表达SAP18可增强HeLa细胞克隆形成、增殖和迁移能力。敲低HPV E6和E7蛋白导致SAP18表达水平下调。这一研究说明HPV通过E6和E7蛋白上调SAP18的表达促进宫颈癌恶性转化、增殖和迁移,SAP18蛋白的高表达不利于宫颈癌患者的生存。更多还原

【Abstract】 To explore the expression of SAP18 in cervical cancer tissues, and to investigate the effect of SAP18 on the proliferation and migration of cervical cancer cells HeLa and its molecular mechanism. TCGA and GTEx database was used to analyze the survival of patients with cervical cancer with differential expression of SAP18, and the expression of SAP18 in cancer and adjacent tissues of cervical cancer patients simultaneously. Immunohistochemistry(IHC) was adopted to observe the tissue distribution of SAP18 in cervical cancer tissues and adjacent normal tissues. After overexpressing SAP18 in HeLa cells, the effect of SAP18 on the proliferation of HeLa cells was identified by cell count kit-8 and soft agar assay. Wound-healing assay was adopted to detect the effect of SAP18 overexpression on cell migration. Specific siRNA was designed to knock down the E6 and E7 proteins of human papillomavirus, followed by Western blot to verify the knockdown level and the expression of SAP18. Result showed that SAP18 was highly expressed in cervical cancer tissues and was negatively correlated with the survival rate of cervical cancer patients simultaneously. Overexpression of SAP18 promoted HeLa cells proliferation and migration. Knockdown of HPV E6/E7 decreased the expression of SAP18. This study suggests that SAP18 is highly expressed in cervical cancer, and is regulated by HPV E6/E7 to promote tumor proliferation and migration, which is not conducive to the survival of cervical cancer patients.更多还原