【摘要】 将鼠李糖乳杆菌(Lactobacillus rhamnosus)ZB1107-01(LR ZB1107-01)与Lactobacillus rhamnosus GG(LGG)商业菌株进行体外抗氧化能力的比较,以DPPH(1,1-二苯-三硝基苯肼)自由基、羟自由基及超氧阴离子自由基清除率为指标,并建立Caco-2细胞氧化损伤模型,通过测定SOD、GSH-Px酶活性,探究LR ZB1107-01对细胞氧化应激的影响。结果表明:菌浓度越高,对3种自由基的清除能力越强,LR ZB1107-01的发酵液对DPPH自由基的清除率显著高于LGG,上清液对羟自由基的清除率略高于LGG,细胞裂解液对超氧阴离子自由基的清除率显著高于LGG;在Caco-2细胞氧化损伤模型中,对SOD、GSH-Px酶活性进行测定,发现LR ZB1107-01可以降低Caco-2细胞的氧化应激损伤,提高氧化应激状态下的抗氧化能力。更多还原

【Abstract】 The antioxidant capacities of Lactobacillus rhamnosus ZB1107-01(LR ZB1107-01) and Lactobacillus rhamnosus GG(LGG) in vitro were compared. Taking free radical scavenging activity of DPPH clearance, hydroxyl clearance and superoxide anion as the indexes and establishing the oxidative damage model of Caco-2 cells, this study investigated the effects of LR ZB1107-01 on oxidative stress of Caco-2 cells by measuring the activities of SOD and GSH-Px. The results show that the higher the concentration of bacteria, the stronger the ability of scavenging the three free radicals. The scavenging capacity of LR ZB1107-01 fermentation liquor on DPPH is significantly higher than that of LGG, the scavenging capacity of supernatant on hydroxyl radical is slightly higher than that of LGG, and the scavenging capacity of cell lysate on superoxide anion free radical is significantly higher than that of LGG. In the model of oxidative damage of Caco-2 cells, the measurement of the enzymatic activity of SOD and GSH-Px shows that LR ZB1107-01 can reduce the oxidative stress damage of Caco-2 cells and increase its antioxidant function under oxidative stress.更多还原