【摘要】 金纳米颗粒所具有的表面拉曼增强(SERS)效应使其在生物医学检测方面有很多潜在的应用.文中分别采用原位合成、物理混合两种方法制备了金纳米复合丝素膜,并通过在丝素蛋白溶液中添加甘油的方法对丝素膜进行韧性、强度及不溶性的改进.利用扫描电镜(SEM)、X射线衍射仪(XRD)、紫外-可见分光度计(UV)对其形貌和结构进行表征.丝素膜表面生成的金纳米颗粒平均粒径约为30 nm,紫外-可见特征吸收峰出现在535 nm处.以4-巯基苯甲酸(4-MBA)进行了拉曼测试.结果表明,采用原位合成方法中两步合成法合成的金纳米复合丝素膜具有良好的SERS效应,可应用于痕量分析检测.更多还原

【Abstract】 Surface-enhanced Raman scattering(SERS) effect of gold nanoparticles has many potential applications in biomedical detection. Here, we synthesized the gold nanocomposite silk film by in situ synthesis and physical mixing, respectively. The toughness, strength and insoluble properties of silk film were improved by adding glycerin to silk fibroin solution. The morphology and mechanism were characterized by scanning electron microscopy(SEM), X-ray diffraction(XRD) and ultraviolet-visible spectrophotometer(UV-vis). The average particle size of the gold nanoparticles synthesized on the silk film is about 30 nm, and the UV-vis characteristic absorption appears at 535 nm. Raman spectroscopy was performed with 4-mercaptobenzoic acid(4-MBA). The results show that the gold nanocomposite silk film synthesized by in situ two-step synthesis has good SERS effect and can be applied to trace analysis detection.更多还原