【摘要】 为明确烟粉虱取食ToCV侵染番茄后其生理防御反应相关基因的变化,通过转录组测序和实时荧光定量PCR技术研究烟粉虱MED隐种取食ToCV侵染番茄和健康番茄6,12,24,48 h,其体内解毒酶、保护酶系统以及基因功能、代谢功能的变化。结果表明,烟粉虱MED隐种取食ToCV侵染番茄不同时间,其体内解毒酶细胞色素P450酶(P450)、谷胱甘肽S-转移酶(GST)、羧酸酯酶(CarE)和保护酶超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)转录水平均发生不同程度的变化;除CAT外,P450、GST、CarE、SOD和POD表达量总体趋于上调,其中发挥主要作用的是P450和SOD。通过对差异表达基因进行GO注释和KEGG功能分析,发现烟粉虱MED隐种取食ToCV侵染番茄12 h,其差异基因的生物学功能和代谢途径与其他时间点不同,该时间点下烟粉虱主要通过溶酶体实现蛋白质分解,而其他时间点下烟粉虱则通过核糖体逐步实现氨基酸到蛋白质的生物合成。综上,烟粉虱取食ToCV侵染番茄可能引起了其解毒酶、保护酶基因表达的改变,并通过溶酶体途径调控自身免疫应对ToCV入侵;其中,12 h可能是烟粉虱与ToCV互作的关键时间点。研究结果有助于进一步阐明烟粉虱取食ToCV获毒后的防御机制,丰富烟粉虱-植物病毒-寄主植物间的互作理论,为田间ToCV的有效防控提供科学依据。更多还原

【Abstract】 To clarify the defense responses of Bemisia tabaci after feeding on ToCV-infected tomato, RNA-Seq analysis and qRT-PCR were performed to explore changes that occurred with the internal detoxification and protective enzyme systems as well as with the gene expression and metabolic functions 6,12,24,48 hours respectively after B. tabaci MED fed on both ToCV-infected and uninfected tomato plants. The results showed that the transcription level of detoxification enzymes P450,GST,CarE and protective enzymes SOD,POD,CAT made different changes when B. tabaci MED feeding on ToCV-infected tomato at different times. The genes expression levels of P450,GST,CarE,SOD and POD tended to be up-regulated with the exception of CAT,among which P450 and SOD played the most major roles. Through GO annotation and KEGG function analysis of DEGs, it was found that biological function and metabolic pathways of DEGs of B. tabaci MED after feeding on ToCV-infected tomato for 12 h were different from other time points. After feeding for 12 h, B. tabaci MED mainly decomposed protein through lysosomes, while biosynthesis of amino acids to proteins were carried out through ribosomes at other times. In conclusion, B. tabaci that feeding on ToCV-infected tomato might have caused changes on the gene expression of detoxification and protective enzymes, and regulated the autoimmune response to ToCV invasion through lysosomal pathway. Among them, 12 hours feeding period might be the critical point for B. tabaci to interact with ToCV. The results here are helpful to further clarify the defense mechanism of B. tabaci toward ToCV infections, enrich the interaction theory of B. tabaci-ToCV-host, and provide a scientific basis for effective control of ToCV in the field.更多还原