【摘要】 目的:探讨miR-1915-3p和Bcl-2 mRNA在结肠癌组织中的表达及其与结肠癌患者临床病理参数和预后的相关性,阐明miR-1915-3p和Bcl-2在结肠癌发生发展中的作用。方法:收集结肠癌组织及癌旁正常黏膜组织标本各40例,应用实时荧光定量聚合酶链式反应(RT-qPCR)技术检测结肠癌组织和癌旁正常黏膜组织中miR-1915-3p和Bcl-2 mRNA表达水平,采用Pearson相关分析法分析miR-1915-3p表达水平与和Bcl-2 mRNA表达水平的相关性,采用χ~2检验分析miR-1915-3p和Bcl-2与结肠癌患者临床病理参数和预后的关系,采用Kaplan-Meier法绘制生存曲线,生存差异行Log-rank检验。结果:RT-qPCR检测,与癌旁正常黏膜组织比较,结肠癌组织中miR-1915-3p表达水平明显降低(P<0.05), Bcl-2 mRNA表达水平明显升高(P<0.05);且结肠癌组织中miR-1915-3p与Bcl-2mRNA表达水平呈负相关关系(r=-0.542,P<0.05)。结肠癌组织中,miR-1915-3p表达水平与肿瘤分化程度和淋巴结转移有明显关联(P<0.05),miR-1915-3p表达水平与患者性别、年龄、肿瘤大小和TNM分期无关联(P>0.05)。结肠癌组织中Bcl-2 mRNA表达水平与TNM分期有关(P<0.05),而与其他临床病理参数无关联(P>0.05)。Kaplan-Meier生存曲线,miR-1915-3p高表达组结肠癌患者总生存期(OS)长于miR-1915-3p低表达组(χ~2=3.641,P<0.05),而Bcl-2 mRNA表达水平与结肠癌患者预后无关联(χ~2=0.571,P>0.05)。结论:结肠癌患者肿瘤组织中miR-1915-3p表达水平明显降低,而Bcl-2 mRNA表达水平明显升高,二者的联合检测有助于结肠癌的诊断和预后评估。更多还原

【Abstract】 Objective:To investigate the expressions of miR-1915-3p and Bcl-2 mRNA in colon cancer tissue and their relationships with the clinicopathological parameters and prognosis of the colon cancer patients,and to clarify the roles of miR-1915-3p and Bcl-2 in the occurrence and development of colon cancer. Methods:A total of 40 colon cancer specimens and paired paracancer normal mucosa tissue were collected for examination of miR-1915-3p and Bcl-2 mRNA expression levels by Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR). The correlation between the miR-1915-3p expression level and Bcl-2 mRNA expression level was analyzed by Pearson correlation analysis. The correlations between the expressions of miR-1915-3p and Bcl-2 and the clinicopathological features and prognosis of the colon cancer patients were analyzed by χ~2 test. The survival curve was drawn by Kaplan-Meier method,and the survival difference was tested by Log-rank test. Results:The RT-qPCR results showed that compared with the adjacent mucosa tissue,the expression level of miR-1915-3p in colorn cancer tissue was decreased significantly(P<0. 05),and the expression level of Bcl-2 mRNA in colon cancer tissue was increased(P<0. 05);there was a significant negative correlation between the expression level of miR-1915-3p and the expression level of Bcl-2 mRNA in colon cancer tissue(r=-0. 542,P<0. 05). The expression lervels of miR-1915-3p in colon cancer tissue was significantly correlated with the degree of tumor differentiation and lymph node metastasis(P<0. 05),but not with gender,age,tumor size and TNM stage(P>0. 05). The expression level of Bcl-2 mRNA in colon cancer tissue was significantly correlated with TNM stage(P<0. 05),but was not related to other clinicopathological features(P>0. 05). The Kaplan-Meier analysis results of survival curve showed that the overall survival(OS)of the colon cancer patients in miR-1915-3p high expression group was lower than that in miR-1915-3p low expression group(χ~2=3. 641,P<0. 05).There was no significant relationship between the expression level of Bcl-2 mRNA and the prognosis of colon cancer patients (χ~2=0. 571,P>0. 05). Conclusion:The miR-1915-3p expression level in tumor tissue of the colorectal cancer patients is decreased,but the expression level of Bcl-2 mRNA is significantly increased. The combined detection of them is useful for the diagnosis and prognosis evaluation of colon cancer.更多还原