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培养液种类和浓度对草履虫个体及数量的影响

Effects of culture medium type and concentration on individual and quantity of paramecium

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【作者】 周琳李鑫卉古红梅

【Author】 ZHOU Lin;LI Xinhui;GU Hongmei;College of Life Science and Agronomy,Zhoukou Normal University;

【通讯作者】 古红梅;

【机构】 周口师范学院生命科学与农学学院

【摘要】 实验选取玉米粒、奶粉、熟鸡蛋黄、小麦和酵母5种材料配制成培养液来培养草履虫.同时观察它们对草履虫种群生长的影响.结果显示:玉米粒、奶粉、熟鸡蛋黄培养液为草履虫理想培养液.配制3种理想材料的不同浓度梯度的培养液,探究适合草履虫生长和繁殖的最适浓度.结果显示:玉米粒培养液的最适浓度为每1000 mL水中加10~20 g玉米粒;熟鸡蛋黄培养液的最适浓度为每60 mL冷开水中加入0.1~0.2 g熟鸡蛋黄;奶粉培养液的最适浓度为每1 000 mL水中加入1~2 g奶粉.这说明培养液种类和浓度对草履虫的生长和繁殖均有不同程度的影响.

【Abstract】 The culture medium was prepared from corn kernel,milk powder,cooked egg yolk,wheat and yeast to cultivate paramecium.At the same time,the effect of culture medium on the growth of paramecium population was observed.The results showed that corn kernel,milk powder and cooked egg yolk were ideal medium for paramecium.The optimal concentration for growth and reproduction of paramecium was studied by preparing the culture medium with different concentration gradient of 3 ideal materials.The results showed that the optimum concentration of corn kernel medium was 10~20 g corn kernel per 1 000 mL water;the optimum concentration of egg yolk medium was 0.1 ~0.2 g cooked egg yolk per60 cold boiled water;the optimum concentration of milk powder medium was 1 ~2 g milk powder per 1 000 mL water.This indicated that the variety and concentration of the culture medium had different effects on the growth and reproduction of paramecium.

【基金】 国家自然科学基金项目“基于形态和分子数据的中国大豆食心虫种群分化研究”(31702046)
  • 【文献出处】 周口师范学院学报 ,Journal of Zhoukou Normal University , 编辑部邮箱 ,2020年05期
  • 【分类号】Q95-33
  • 【下载频次】619
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