【摘要】 目的探讨慢性乙型肝炎(CHB)患者外周血Tfh细胞相关表达分子CXCR5、CXCL13、IL-21和TGF-β2的表达水平,分析其在CHB发生、发展中的作用。方法选取2018年10月～2019年3月广州中医药大学第一附属医院收治的CHB患者50例,再选取同期健康体检人员30例作为对照组,采用ELISA法检测两组受试者外周血中CXCR5、CXCL13、IL-21和TGF-β2的表达水平,荧光定量PCR检测HBV DNA,化学发光法检测HBs Ag、HBe Ag,酶速率法检测AST和ALT等指标。结果 CHB患者组CXCR5、CXCL13、IL-21和TGF-β2表达明显高于健康对照组(P <0.01),但与HBV DNA、HBs Ag、AST、ALT等之间无相关性(P>0.05)。CHB患者中HBe Ag阴性组CXCR5、CXCL13、IL-21和TGF-β2的表达比HBe Ag阳性组升高,差异无统计学意义(P>0.05)。结论 Tfh细胞及其功能相关分子IL-21和TGF-β2参与了HBV感染后相关的免疫应答反应,可以作为不同免疫状态下免疫损伤的预测指标。更多还原

【Abstract】 Objective To discuss the expression level of chronic-hepatitis-B-related expression molecule CXCR5,CXCL13,IL-21,and TGF-β2 in peripheral blood of patients with chronic hepatitis B( CHB) and to analyze the function of expression molecule in the development of CHB. Methods The peripheral blood of 50 patients with CHB in The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine were chosen to be compared with the peripheral blood of 30 healthy individuals,controlled group,drawn from the physical examination. Expression levels of CXCR5,CXCL13,IL-21,and TGF-β2 in peripheral blood of participating individuals were evaluated through ELISA. HBV DNA of the peripheral blood was tested through PCR. HBs Ag and HBe Ag were indicated through CLEIA. The levels of AST,ALT,etc. were accessed through continuous monitoring assay. Results The expression levels of CXCR5,CXCL14,IL-21,and TGF-β2 in patients with CHB were significantly higher than the controlled group of healthy individuals( P <0. 01),but there was no critical correlation between expression levels and HBV DNA,HBs Ag,AST,ALT,etc.( P > 0. 05). The expression levels of CXCR5,CXCL13,IL-21,and TGF-β2 in HBe Ag negative group of patients with CHB were slightly higher than the expression levels of CXCR5,CXCL13,IL-21,and TGF-β2 in HBe Ag positive group of patients,but the difference of expression level in two groups had no statistical significance( P > 0. 05). Conclusion Follicular helper T cells,IL-21,and TGF-β2 are associated with the immune response of HBV infection. And they might be used as a predictor of immune damage in different immune states of patients with CHB.更多还原