【摘要】 背景:在临床治疗中,创伤性神经损伤伴有骨折的患者表现出较单纯骨折愈合加速且骨痂过量生长,甚至在肌肉中出现异位骨化,严重影响这类骨折的治疗效果。对于影响失神经后骨折愈合加速的具体原因和机制,目前并不清楚。目的:探索成纤维细胞生长因子受体3抑制剂在骨折愈合过程中的作用及表达变化规律。方法:实验方案经兰州大学第二医院动物实验伦理委员会批准。选用60只雌性SD大鼠,制作坐骨神经损伤的胫骨横行骨折模型,随机分为实验组及对照组2组。实验组造模后腹腔注射成纤维细胞生长因子受体3阻滞剂;对照组造模后给等剂量生理盐水。分别于造模后4,7,10,14,21 d拍X射线片后取胫骨大体标本(每个时间点6只),取胫骨进行苏木精-伊红染色、Masson三色法染色组织学观察;计算大鼠胫骨骨细胞密度和骨小梁密度;测定胫骨组织纤维率。结果与结论:①两组大鼠胫骨X射线观察差别不显著;②苏木精-伊红染色、Masson三色法染色结果显示实验组修复效果比对照组较好;③2组大鼠胫骨骨细胞密度、骨小梁密度及胫骨组织纤维率在7-14 d有明显差别(实验组>对照组);④抑制成纤维细胞生长因子受体3在周围神经失神经情况下能够加快骨折愈合,促进骨痂的塑形。成纤维细胞生长因子受体3在骨折后7-14 d时间段表达最为活跃。更多还原

【Abstract】 BACKGROUND: In clinical treatment, patients with traumatic nerve injury and fractures show accelerated fracturehealing and excessive osteophyte growth, and even heterotopic ossification in the muscle, all of which seriously affect the therapeutic efficacy on such fractures. The specific causes and mechanisms for the acceleration of fracture healing after denervation are currently unclear. OBJECTIVE: To investigate the role and expression of fibroblast growth factor receptors 3(FGFR3) inhibitor in the process of fracture healing. METHODS: The study protocol was approved by the Animal Ethics Committee of Second Hospital of Lanzhou University. Sixty female Sprague-Dawley rats were used to make a transverse humeral fracture model of sciatic nerve injury. They were randomly divided into two groups, experimental group and control group. The experimental group received intraperitoneal injection of FGFR3 blocker; the control group received an equal dose of normal saline. The X-ray films were taken at 4, 7, 10, 14 and 21 days after surgery, and tibia specimens for six animals were subsequently taken at each time point, followed by histological observations using hematoxylin-eosin staining and Masson’s trichrome staining. Osteocyte density and trabecular bone density of the rat tibia were calculated; and the fiber rate of the tibia was determined. RESULTS AND CONCLUSION: There were no significant differences in the X-ray findings of the tibia between the two groups. The experimental group had better bone repair than the control group, shown by hematoxylin-eosin staining and Masson’s trichrome staining. Osteocyte density, trabecular bone density, and fiber rate of the rat tibia were significantly higher in the experimental group than the control group at 7-14 days after treatment. Inhibition of FGFR3 can accelerate fracture healing and promote the shaping of callus in the case of peripheral nerve denervation. FGFR3 is most active at 7-14 days after fracture.更多还原