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菠萝蛋白酶金纳米簇模拟酶活性比色法检测Hg2+

Colorimetric detection of Hg2+ with bromelain-AuNCs peroxidase-like activity

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【作者】 刘士蒙李嘉倩吕昌银刘然李诗雅杨桂英

【Author】 LIU Shi-meng;LI Jia-qian;Lü Chang-yin;LIU Ran;LI Shi-ya;YANG Gui-ying;College of Public Health,University of South China,Key Laboratory of Hengyang for Health Hazard Factors Inspection and Quarantine;

【通讯作者】 吕昌银;

【机构】 南华大学公共卫生学院衡阳市健康危害因子检验检疫新技术研究重点实验室

【摘要】 制备了具有模拟过氧化物酶活性的菠萝蛋白酶-金纳米簇,可催化3,3′,5,5′-四甲基联苯胺(TMB)-H2O2体系显色。因此,构建了Bromelain-AuNCs-TMB-H2O2-Hg2+比色传感平台,并对该体系的催化显色反应机制进行了研究。本方法检测Hg2+的线性范围为6.25 nmol/L~3.5μmol/L,检出限为4.3 nmol/L。本方法成本低,简便可行,选择性好,为环境水样中的Hg2+检测提供了一种新方法。

【Abstract】 Bromelain-gold nanoclusters(AuNCs) was successfully prepared that possess simulated peroxidase activity,it catalyzes the color development of the 3,3′,5,5′-tetramethylbenzidine(TMB)-H2O2 system.Therefore,Bromelain-AuNCs-TMB-H2O2-Hg2+ colorimetric sensing platform was designed and researched the catalytic color reaction mechanism in the system.The linear range of detection of Hg2+ extends from 6.25 nmol/L~3.5 μmol/L,and the lower detection limit is 4.3 nmol/L.The method was cost-effective,simple and feasible,with high selectivity,thus serving as a new strategy for Hg2+ detection in environmental water samples.

【基金】 国家自然科学基金(81473021);衡阳市重点实验室项目资助(2018KJ110)
  • 【文献出处】 应用化工 ,Applied Chemical Industry , 编辑部邮箱 ,2020年05期
  • 【分类号】O657.3;X832
  • 【被引频次】1
  • 【下载频次】251
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