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己酸菌发酵条件优化及重金属Pb对己酸菌繁殖代谢的影响

Optimizing Fermentation Conditions and the Effect of Pb on Caproic Acid Bacteria Reproduction and Metabolism

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【作者】 景万星; 商美; 魏萍; 翟欢; 肖启荣; 姚继承;

【Author】 JING Wanxing;SHANG Mei;WEI Ping;ZHAI Huan;XIAO Qirong;YAO Jicheng;Wuhan Jiacheng Biotechnology Co., Ltd;Hubei Province Food Fermentation Technology Engineering Center;

【通讯作者】 姚继承;

【机构】 武汉佳成生物制品有限公司; 湖北省食品发酵工程技术研究中心;

【摘要】 研究通过单因素实验确定了影响菌株CB9产己酸的因素条件,再利用正交试验确定了各因素的最优组合,即大米粉0.6%,酵母膏1.0%,碳酸钙0.3%,乙醇1.5%。此条件下,己酸的产量为4.84g/L。通过固液两种发酵方式研究了重金属Pb对己酸菌繁殖代谢的影响,发现随着重金属Pb含量的增加,无论是己酸的产量还是己酸菌的浓度都会不断地下降。

【Abstract】 In this study, the factors affecting the production of caproic acid by the strain CB9 were determined by single factor experiments, and then the orthogonal test was used to determine the optimal combination of factors, namely rice powder 0.6%, yeast extract 1.0%, calcium carbonate 0.3%, ethanol 1.5%. Under this condition, the yield of caproic acid was 4.84 g/L. The effects of Pb on caproic acid bacteria reproduction and metabolism were studied by solid-state fermentation and liquid fermentation. It was found that both the yield of caproic acid and the concentration of caproic acid bacteria decreased with the increase of Pb content.

【关键词】 己酸菌; 窖泥; 重金属; 铅Pb;
【Key words】 caproic acid bacteria; pit mud; heavy metal; lead(Pb);
  • 【文献出处】 酿酒 ,Liquor Making , 编辑部邮箱 ,2020年05期
  • 【分类号】TS262.3
  • 【下载频次】167
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