【摘要】 为了确定健康育肥猪肠道变形杆菌的种类和遗传进化规律,试验使用培养组学技术来分离培养健康育肥猪肠道变形杆菌,纯化培养扩繁后的细菌通过扩增16S rRNA基因片段并测序鉴定菌种,最终测序结果共鉴定出6个菌种包括:10株奇异变形杆菌、5株变形杆菌、4株表皮葡萄球菌、2株腐生葡萄球菌和3株地衣芽孢杆菌。通过16S rRNA序列在NCBI上与Gen Bank中公布的15株奇异变形杆菌参考株进行同源性对比分析,同源性为99.8%～100%,系统发育树分析发现,分离菌株H1-H15与15株参考株亲缘关系较近,同源性均为99.9%,其中11株奇异变形杆菌,4株普通变形杆菌;奇异变形杆菌和普通变形杆菌对于氟苯尼考、头孢曲松高度敏感;阿莫西林、庆大霉素耐药。通过发育树分析和同源性比对分析说明了健康育肥猪肠道奇异变形杆菌和普通猪源性变形杆菌的遗传进化规律;15株变形杆菌的综合药敏实验结果为规模化养猪场变形杆菌起到了防控作用。更多还原

【Abstract】 In order to determine the species and genetic evolution of Proteus mirabilis in healthy fattening pig intestine,culture histology technology was used to isolate and culture Proteus mirabilis in healthy fattening pig intestine. After purification,the expanded bacteria were identified by amplifying 16 S rRNA gene fragments and sequencing. The final sequencing results identified six strains including 10 Proteus mirabilis,5 Proteus mirabilis and 4 strains of surface. Staphylococcus dermatis,2 Staphylococcus saprophyticus and 3 Bacillus licheniformis strains. The homology of 16 S rRNA sequence between NCBI and 15 reference strains of Proteus mirabilis published in Gen Bank was 99.8%-100%. Phylogenetic tree analysis showed that H1-H15 was closely related to15 reference strains with 99.9% homology,including 11 Proteus mirabilis,4 Proteus vulgaris,Proteus mirabilis and Proteus vulgaris.Bacteria are highly sensitive to florfenicol and ceftriaxone,and resistant to amoxicillin and gentamicin. The genetic evolution rule of Proteus mirabilis and common porcine Proteus in healthy fattening pigs was illustrated by developmental tree analysis and homology comparison analysis. The comprehensive susceptibility test of 15 strains of Proteus played a role in the prevention and control of Proteus in large-scale pig farms.更多还原