【摘要】 本工作设计并合成头孢菌素酶特异性底物,研究了头孢菌素酶对该底物的催化反应机制,结合共振能量转移信号放大策略,构建了一种用于氯霉素检测的新型免疫传感器。为了实现目标物的快速分离和提高检测灵敏度,在免疫磁珠表面修饰抗体,同时在聚苯乙烯微球上修饰头孢菌素酶和抗原,利用免疫竞争法实现了对氯霉素的定量检测。该研究构建的新型传感器具有高稳定性、高灵敏度等优点,为即时检测提供了新的思路。更多还原

【Abstract】 An Amp Cephalosporinase(AmpC)-specific substrate was designed and synthesized. The catalytic reaction mechanism of AmpC on this substrate was studied. Combined with fluorescence resonance energy transfer signal amplification strategy, a novel enzymatic-based immunosensor was constructed for chloramphenicol detection. For rapid separation of the Target and enhancement of the detection sensitivity, the capture antibody was conjugated on Immuno-magnetic beads(IMBs-Ab), and the AmpC and antigen(chloramphenicol-ovalbumin, cap-ova) was conjugated to polystyrene microspheres simultaneously to prepare PS-AmpC/Ag. The quantitative of cap was achieved by using the immune competition method. This approach has the advantage of high stability and sensitively, which provides a new idea for point-of-care testing.更多还原