【摘要】 脯氨酸类衍生物结构独特,鲜有报道利用核磁共振（nuclear magnetic resonance, NMR）技术对氨基酸的手性进行鉴别.利用多种NMR技术:¹H NMR、¹H-¹H同核位移相关谱（¹H-¹H COSY）、¹H-¹H质子全相关谱（¹H-¹HTOCSY）、¹H-¹H核Overhauser效应谱（¹H-¹H NOESY）、¹³C NMR、无畸变的极化转移增强法（DEPT135°）、¹H-¹³C检出¹H的异核单量子相干(¹H-¹³C HSQC)和¹H-¹³C检出¹H的异核多键相关(¹H-¹³C HMBC),对脯氨酸类N-酰胺衍生物两种构象异构体的¹H和¹³C NMR进行了全归属,确定了室温下在二甲基亚砜（DMSO）中L型和D型的顺反异构体以相同的比例同时存在.更多还原

【Abstract】 Proline derivatives have unique structures, and few reports have been published on the chiral identification of amino acids by nuclear magnetic resonance（NMR）. The structures of two proline N-amide conformation isomers were analyzed in detail using ¹H NMR, ¹H-¹H correlated spectroscopy（¹H-¹H COSY）, ¹H-¹H total correlation spectroscopy（¹H-¹H TOCSY）, ¹H-¹H nuclear overhauser effect spectroscopy（¹H-¹H NOESY）, ¹³C NMR, distortionless enhancement by polarization transfer（DEPT135°）, ¹H-¹³C ¹H detected heteronuclear single quantum coherence(¹H-¹³C HSQC) and ¹H-¹³C ¹H detected heteronuclear multiple-bond correlation(¹H-¹³C HMBC) techniques, and the positions of ¹H and ¹³C NMR peaks of the derivatives were assigned. The co-existence of L-and D-type cis-trans isomers in dimethylsulfoxide（DMSO） at room temperature were determined.更多还原