【摘要】 建立同时测定鱼、虾中金刚烷胺、金刚乙胺、美金刚和吗啉胍4种抗病毒药物残留的分散固相萃取—超高效液相色谱—串联质谱法。样品采用1%的三氯乙酸和1%乙酸乙腈提取(1∶9,体积比),经PSA粉、C18粉、中性氧化铝分散固相萃取净化,内标法定量。检测结果表明,4种抗病毒药物的检出限均低于0.8μg/kg,定量限均低于1.0μg/kg,空白鱼、虾中分别添加1.0、10.0、50.0μg/kg,4种抗病毒药物的回收率为86.0%～98.5%,相对标准偏差小于8%。该方法基质干扰小、灵敏度高、回收率好,可用于日常鱼、虾样品中抗病毒类药物的残留检测。更多还原

【Abstract】 An efficient ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method was established for the simultaneous analysis of residues of 4 antiviral drugs( amantadine, rimantadine, morpholine, and memantine) in fish and shrimp. Samples were extracted with 1% trichloroacetic acid and 1% acetic acid acetonitrile(1∶9, V/V), purified by PSA powder, C18 powder, neutral alumina dispersed solid phase extraction. Quantifification of the analytes was achieved by UPLC-MS/MS using the internal standard method. The limit of detection(LOD) and limit of quantifification(LOQ) of the method were lower than 0.8 μg/kg and 1.0 μg/kg for all the antiviral drugs, respectively. The average recoveries of 4 antiviral drugs in blank fish and shrimp at spiked levels of 1.0, 10.0, and 50.0 μg/kg ranged from 86.0% to 98.5%, with relative standard deviation(RSD) of less than 8%. The method is featured by small matrix interference, high sensitivity and good recovery rate, and can be used for residue detection of antiviral drugs in daily fish and shrimp samples.更多还原